使用膜生物反应器培养浮游厌氧铵氧化（Anammox）细菌

MicrobesEnviron.Vol.28,No.4,436–443,2013https://www.jstage.jst.go.jp/browse/jsme2doi:10.1264/jsme2.ME13077CultivationofPlanktonicAnaerobicAmmoniumOxidation(Anammox)BacteriaUsingMembraneBioreactorMAMORUOSHIKI1,TAKANORIAWATA2,TOMONORIKINDAICHI2,HISASHISATOH1,andSATOSHIOKABE1*1DivisionofEnvironmentalEngineering,FacultyofEngineering,HokkaidoUniversity,North13,West-8,Sapporo,Hokkaido060–8628,Japan;and2DepartmentofCivilandEnvironmentalEngineering,GraduateSchoolofEngineerging,HiroshimaUniversity,1–4–1Kagamiyama,Higashihiroshima739–8527,Japan(ReceivedJune8,2013—AcceptedAugust11,2013—PublishedonlineNovember8,2013)Enrichmentculturesofanaerobicammoniumoxidation(anammox)bacteriaasplanktoniccellsuspensionsareessentialforstudyingtheirecophysiologyandbiochemistry,whiletheircultivationisstilllaborious.Thepresentstudyaimedtocultivatetwophylogeneticallydistinctanammoxbacteria,“CandidatusBrocadiasinica”and“Ca.Scalinduasp.”intheformofplanktoniccellsusingmembranebioreactors(MBRs).TheMBRswerecontinuouslyop