蜈蚣草总黄酮提取工艺的优选

蜈蚣草总黄酮提取工艺的优选周向军,杨宗琪,景红艳,高义霞(1.天水师范学院生命科学与化学学院甘肃,天水741001)��摘要:采用水浴法提取总黄酮,以蜈蚣草为原料、总黄酮得率为考察指标,通过正交试验研究乙醇浓度、提取时间、提取温度及料液比对提取蜈蚣草总黄酮得率的影响。结果表明,各因素对总黄酮提取率的影响程度为�乙醇浓度>提取时间>提取温度>料液比;最佳的提取工艺条件为:乙醇浓度60%,料液比1�15,提取温度60,每次3h,提取3次,在此条件下提取的总黄酮得率为6.15%。关键词:蜈蚣草;总黄酮;提取工艺中图分类号:Q946.91��文献标志码:A��文章编号:1005-8141(2010)03-0210-02StudyonOptimizationofTotalFlavonoidsExtractionTechnologyinPterisVittataL.ZHOUXiang-jun,YANGZong-qi,JINGHong-yan,GAOYi-xia(SchoolofLifeScience,TianshuiNormalCollege,Tianshui741001,China)Abstract:TheethanolextractiontechnologyoftotalflavonoidsinPterisvittataL.wasoptimizedbyorthogonalexperiments.Theexperimentsre�sultsshowedthattheorderofdifferentfactorsaffectingextractionratewasethanol,extractiontime,extractiontemperateandrateofsolid/liquid.Theoptimalexperimentalparameterswereethanol60%,extractiontemperate60,rateofsolid/liquid1�15,extractiontime3h,andthreetimes.Underthiscondition,theyieldoftotalflavonoidswas6.15%.Keywords:PterisvittataL.;totalflavonoids;extractiontechnology��