亚硝化细菌的分离及初步鉴定_廖雪义

[14]VitielloL,BockholdK,JoshiPB,etal.Transfectionofculturedmyo-blastsinhighserumconcentrationwithDODAC:DOPEliposomes[J].GeneTher,1998,5(10):1306-1313.[15]李克深.阳离子脂质体介导的转染:血清对于表达及转染效率的影响[J].国外医学:遗传学分册,1995,18(5):273-274.[16]邹东霆,李冰.脂质体介导转染培养细胞的基因表达及转染程序的简化[J].广州医学院学报,2005,33(5):50-52.[17]DelteilC,TeissiéJ,RolsMP.Effectofserumoninvitroelectricallyme-diatedgenedeliveryandexpressioninmammaliancells[J].BiochimBiophysActa,2000,1467(2):362-368.[18]MorimotoK,KondoM,KawaharaK,etal.Advancesintargetingdrugdeliverytoglomerularmesangialcellsbylongcirculatingcationicliposomesforthetreatmentofglomerulonephritis[J].PharmRes,2007,24(5):946-954.[19]WeissDJ,BonneauL,LiggittD.Useofperfluorochemicalliquidallowsearlierdetectionofgeneexpressionanduseoflessvectorinnormallunganden-hancesgeneexpressioninacutelyinjuredlung[J].MolTher,2001,3(5Pt1):734-745.亚硝化细菌的分离及初步鉴定廖雪义1,蓝荣2(1.襄樊学院化学与生物科学系,湖北襄樊441053;2.广东省中山市杨氏南北鲜果有限公司,广东中山528451)摘要:目的:为了使短程硝化—反硝化生物脱氮工艺能更有效地应用于含氮污废水的处理,需分离出高效的亚硝化细菌。方法:采用亚硝化细菌富集培养基选择培养和硅胶平板分离法,通过