生物硫铁生成菌的选育及其在重金属废水处理中的应用

http://www.cibj.com/应用与环境生物学报ChinJApplEnvironBiol2012，18(1):115~1212012-02-25DOI:10.3724/SP.J.1145.2012.00115IsolationofaBiologicalIronSulfideComposites-producingStrainandItsApplicationinEmergencyTreatmentofHeavyMetalWastewater*LUOLihui1,2,XIEYifei1,LIUQinghua1,TANZhouliang1&LIXudong1**(1ChengduInstituteofBiology,ChineseAcademyofSciences,Chengdu610041,China)(2GraduateUniversityofChineseAcademyofSciences,Beijing100049,China)AbstractAbstractAstrainofsulfatereducingbacteria,whichcouldgeneratebiologicalironsulfidecomposites(BISC),wasisolatedbyusingHungateanaerobictechniqueandnamedasSRB2.TheresearchonitsphysiologicalcharacteristicsandcultivationtechniquesshowedthatstrainSRB2appearedasGram-negative,slightlycurvedrods,andsodiumlactatewasconsideredastheoptimumcarbonsource.ThepHrangewasfrom5.0to9.0(anoptimumpHof7.0)andtheoptimumtemperaturewas35℃.ThecontentsofBISCandsulfidereachedthemaximumvaluesof2.85gL-1and358.048mgL-1onthethirdday,respectively.TheresultsoforthogonalexperimentshowedthattheoptimalmediumforgeneratingBISCwassodiumlactate9.0gL-1,combinationnitrogen1.5gL-1andferroussulfate10.0gL-1.Thesequenceanalysisof16SrDNAindicatedthatstrainSRB2wascloselyrelatedtothetypestrainofDesulfovibriodesulfuricansstrain734,with99%sequencesimilarity.TheBISCappliedinwastewatertreatmentcouldremoveheavymetalsCu2+,Pb2+andCd2+,withremovalratesabove99.8%for2minutes.Therefore,greatprospectsexistfortheapplicationofBISCintheemergencytreatmentofheavymetalcontaminatedwastewaterandheavymetalpollutioncontrol.Fig7,Tab2,Ref20KeywordsKeywordssulfatereducingbacteria;biologicalironsulfidecomposites(BISC);Desulfovibrio;heavymetalwastewatertreatment;cultivationtechnologyCLCX703.1:X172生物硫铁生成菌的选育及其在重金属废水处理中的应用*罗丽卉1,2谢翼飞1刘庆华1谭周亮1李旭东1**（1中国科学院成都生物研究所成都610041）（2中国科学院研究生院北京100049）摘要为开发高效经济、环境友好的重金属废水处理技术，采用亨盖特（Hungate）厌氧技术分离出一株产生物硫铁复合材料的硫酸盐还原菌（Sulfatereducingbacteria，SRB），命名为SRB2，并对该菌株进行了生理特性、培养工艺和废水处理研究.菌体杆状稍有弯曲，革兰氏阴性菌，最佳碳源为乳酸钠.pH范围5.0~9.0，最适pH7.0，最适温度为35℃，培养3d生物硫铁量和硫化物含量能达最大值，分别为2.85gL-1、358.048mgL-1.正交试验结果表明，