锰砂滤膜中的细菌鉴定及滤膜组分分析

第34卷第8期中国电机工程学报Vol.34No.8Mar.15,20142014年3月15日ProceedingsoftheCSEE©2014Chin.Soc.forElec.Eng.1285DOI：10.13334/j.0258-8013.pcsee.2014.08.010文章编号：0258-8013(2014)08-1285-06中图分类号：TM621锰砂滤膜中的细菌鉴定及滤膜组分分析关晓辉，吴飞飞，王子闯，鲁敏，徐小惠(东北电力大学化学工程学院，吉林省吉林市132012)IdentificationofBacteriainFilterMembraneofMatureManganeseSandandAnalysisofConstituentsofFilterMembraneGUANxiaohui,WUfeifei,WANGzichuang,LUMin,XUxiaohui(SchoolofChemicalEngineering,NortheastDianliUniversity,Jilin132012,JilinProvinve,China)ABSTRACT:Thestudywasdesignedtorevealtheremovalmechanismofironandmanganesebymembranefilterofmaturemanganesesandandtoobtainhighlyeffectivestrainstoremoveironandmanganese.Basedonbacteriaseparationfromactivemembraneonthesurfaceofmaturemanganesesandandbacteriaidentificationby16SrDNAsequencing,thestudytestedtheironandmanganeseremovalcapacityofidentifiedstrainsbycatalyzedoxidationinwater,andtheremovalcapacityofmaturemanganesesandafterenlargingcultivationandinoculation.ThesurfacematerialsofmembranefilterofmanganesesandwereanalyzedbyX-rayphotoelectronspectrometry(XPS)aswell.ThestudyshowedthattheseparatedbacteriawereStenotrophomonasandBrevibacillusagri,andbothstrainshavebettercatalyzedoxidationcapacitiesforFe2+andMn2+.Twentydaysafterenlargingcultivationandinoculation,thefiltermaterialsofmanganesesandtendedtobematureandcouldremoveFe2+andMn2+effectively.TheXPSresultsshowedthattheconstituentsofactivemembraneonthesurfaceofmaturemanganesesandwereFe2O3,MnO2,Mn2O3,bacteriaandorganisms.KEYWORDS:maturemanganesesand;bacteriaidentification;catalyzedoxidation;ironremoval;manganeseremoval摘要：为进一步揭示成熟锰砂滤膜除铁锰的机制并获取高效除铁锰菌株，在进行成熟锰砂表面活性滤膜中细菌分离及16SrDNA测序鉴定的基础上，考察了鉴定菌株的催化氧化水中铁锰的能力和菌株经扩大培养并接种后培养成熟的锰砂滤料去除铁锰的情况，并利用X射线光电子能谱(XPS)对锰砂滤膜表面物质进行了分析。研究表明，分离出的细菌分别属于寡养单胞菌、土壤短芽孢杆菌，这2株细菌对水中Fe2+和Mn2+都具有较好的催化氧化能力；菌株经扩大培养基金项目：国家重点基础研究发展计划项目(973项目)(2007CB206904)。TheNationalBasicResearchProgramofChina(973Program)(2007CB206904).并接种于锰砂滤料表面培养20d后滤料趋于成熟，成熟的锰砂滤料可有效去除水中的铁、锰；XPS分析表明，去除水中铁锰的成熟锰砂表面活性滤膜主要由Fe2O3、MnO2、Mn2O3和细菌及有机物等构成。关键词：成熟锰砂；细菌鉴定；催化氧化；除铁；除锰