利用两级UASB消化处理实现木薯燃料乙醇的水循环型生产_英文_孙付保

BIOTECHNOLOGYANDBIOENGINEERINGChineseJournalofChemicalEngineering,18(5)837—842(2010)Water-recycledCassavaBioethanolProductionIntegratedwithTwo-stageUASBTreatment*SUNFubao(孙付保),MAOZhonggui(毛忠贵)**,ZHANGJianhua(张建华),ZHANGHongjian(张宏建),TANGLei(唐蕾),ZHANGChengming(张成明),ZHANGJing(张静)andZHAIFangfang(翟芳芳)KeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,Wuxi214122,ChinaFermentationandEcologicalEngineeringLaboratory(FEEL),SchoolofBiotechnology,JiangnanUniversity,Wuxi214122,ChinaAbstractConsideringlimitedsuccessintarget-hittingdischargefromalcoholindustry,ourattentionwasdirectedtowardarecyclinguseofdistilleryspentwash(DS)incassavabioethanolproductionbyusingatwo-stageup-flowanaerobicsludgeblanketbioremediation(TS-UASBB).WiththeTS-UASBB,24SO−,COD,NandPintheeffluentfromtheDSdegradedsignificantlyandtheirconcentrationswerekeptat0.2g·L−1,2.0g·L−1,1.0g·L−1and15mg·L−1,respectively,in13batchprocessesforwater-recycledethanolfermentation.Withtheeffluentuseddirectlyasdilu-tionwater,noheat-resistantbacteriawerefoundalive.Thethirteen-batchethanolproductionindividuallyachieved10%after48hfermentation.Thestarchutilizationratioandtotalsugarconsumptionwere90%and99.5%,respec-tively.Thenovelwater-recycledbioethanolproductionprocesswithethanolfermentationandTS-UASBBhasaconsiderablepotentialinotherstarchyandcellulosicethanolproduction.Keywordscassava,bioethanolproduction,thermophilicandmesophilic,up-flowanaerobicsludgeblanket,dis-tilleryspentwash,recycleandreuse1INTRODUCTIONCassava(Manihotesculenta),alsocalledyucaormanioc,isaperennialwoodyshrubofEuphorbiaceae(spurgefamily),whichisanativeofSouthAmericaandgrowsintropicalandsubtropicalareas.Asthethirdlargestsourceofcarbohydratesforhumanfoodintheworld,thecassavahasbecomeagoodstarchymaterialinfoodandfermentationindustry.InChina,cassavabasedbioethanolproductionreachesalmost1milliontonsperyear,approximatelyaccountingfor60%ofthetotalbioethanoloutput.Forproductionof1Lethanol,8-15Ldistilleryspentwash(DS),alsoknownasstillage,vinasseordistilleryslop,is