Francis-AOA发现

10.1128/AEM.00402-06.2006,72(8):5643.DOI:Appl.Environ.Microbiol.CriddleandChristopherA.FrancisHee-DeungPark,GeorgeF.Wells,HyokwanBae,CraigS.inWastewaterTreatmentPlantBioreactorsOccurrenceofAmmonia-OxidizingArchaeahttp://aem.asm.org/content/72/8/5643Updatedinformationandservicescanbefoundat:Theseinclude:REFERENCEShttp://aem.asm.org/content/72/8/5643#ref-list-1Thisarticlecites20articles,8ofwhichcanbeaccessedfreeat:CONTENTALERTSmore»articlescitethisarticle),Receive:RSSFeeds,eTOCs,freeemailalerts(whennewhttp://journals.asm.org/site/misc/reprints.xhtmlInformationaboutcommercialreprintorders:http://journals.asm.org/site/subscriptions/TosubscribetotoanotherASMJournalgoto:onOctober10,2014byguesthttp://aem.asm.org/DownloadedfromonOctober10,2014byguesthttp://aem.asm.org/DownloadedfromAPPLIEDANDENVIRONMENTALMICROBIOLOGY,Aug.2006,p.5643–5647Vol.72,No.80099-2240/06/$08.00�0doi:10.1128/AEM.00402-06Copyright©2006,AmericanSocietyforMicrobiology.AllRightsReserved.OccurrenceofAmmonia-OxidizingArchaeainWastewaterTreatmentPlantBioreactorsHee-DeungPark,1GeorgeF.Wells,1HyokwanBae,2CraigS.Criddle,1andChristopherA.Francis3*EnvironmentalEngineeringandScienceProgram,StanfordUniversity,Stanford,California943051;SchoolofEnvironmentalScienceandTechnology,PohangUniversityofScienceandTechnology,Pohang,SouthKorea790-7842;andDepartmentofGeologicalandEnvironmentalSciences,StanfordUniversity,Stanford,California943053Received17February2006/Accepted22May2006Wereportmolecularevidencethatammonia-oxidizingarchaea(AOA)occurinactivatedsludgebioreactorsusedtoremoveammoniafromwastewater.UsingPCRprimerstargetingarchaealammoniamonooxygenasesubunitA(amoA)genes,weretrievedandcompared75sequencesfromfivewastewatertreatmentplantsoperatingwithlowdissolvedoxygenlevelsandlongretentiontimes.Allofthesesequencesshowedsimilaritytosequencespreviouslyfoundinsoilandsediments,andtheyweredistributedprimarilyinfourmajorphylogeneticclusters.OneoftheseclusterscontainedvirtuallyidenticalamoAsequencesobtainedfromallfiveactivatedslud