SBAR中同步脱氮好氧颗粒污泥的菌种特性------污泥细菌和微观结构

中国环境科学2005,25(2)：218~221ChinaEnvironmentalScienceSBAR中同步脱氮好氧颗粒污泥的菌种特性杨凤林1*,王芳1,张兴文1,柳丽芬1,齐爱玖1,宫正2(1.大连理工大学环境与生命学院,辽宁大连116023；2.辽宁师范大学生物系,辽宁大连116029)摘要：采用气提式内循环间歇反应器进行好氧颗粒污泥培养,用分批培养法对好氧颗粒污泥中具有脱氮作用的菌种进行分离纯化.对各菌种进行了鉴别,通过培养过程中对氧气条件的控制,考察了各菌种反硝化生长特性和需氧性.结果表明,好氧颗粒污泥纯化培养可得到异养硝化-好氧反硝化菌以及兼性亚硝化菌和硝化菌,分离得到的反硝化菌在有氧反硝化条件下能够生长,因此好氧颗粒污泥中微生物具有多样性和较强的适应性,从侧面体现出好氧颗粒污泥微观结构的复杂性.关键词：好氧颗粒污泥；内循环间歇反应器；同步脱氮；异养硝化-好氧反硝化中图分类号：X17文献标识码：A文章编号：1000-6923(2005)02-0218-04CharacteristicofthestrainofsimultaneousdenitrificationbacteriaaerobicgranulesludgeinSBAR.YANGFeng-lin1*,WANGFang1,ZHANGXing-wen1,LIULI-fen1,QIAi-jiu1,GONGZheng2(1.SchoolofEnvironmentalandBiologicalScienceandTechnology,DalianUniversityofTechnology,Dalian116023,China；2.DepartmentofBiology,LiaoningNormalUniversity,Dalian116029,China).ChinaEnvironmentalScience,2005,25(2)：218~221Abstract：Aerobicgranulesludgewascultivatedadoptinginternal-circulatesequencingbatchairliftreactor;andbacteriastrainpossessingdenitrificationactioninaerobicgranulessludgewerepurifiedseparatelyusingbatchwttivationtechnique.Eachbacteriastrainwasdistinguished;throughcontrollingoxygenconditioninthecultivatingprocess,thedenitrationgrowthcharacteristicsandaerobicpropertyofeachbacteriastrainwereinspected.Aerobicgranulesludgepurifiedcultivationcouldobtaindifferentlycultivatednitration-aerobicdenitrified,facultativesubritrifierandnitrifier.Theseparatingobtaineddenitrifiercouldgrowunderaerobicdenitrationcondition;therefore,themicrobeinaerobicgranulesludgepossesseddiversityandstrongeradaptability,andembodieddefinitelythecomplexityofthesludgemicrostructure.Keywords：aerobicgranularsludge；internalcirculatesequencingbatchairliftreactor；simultaneousdenitrification；differentlycultivatednitration-aerobicdenitrification近年来,关于好氧颗粒污泥的培养条件、形成机理、结构特性及降解功能的研究成为众多学者关注的热点[1-3].好氧颗粒污泥是以颗粒状形式存在的微生物聚集体,其粒径及密实度不但影响颗粒污泥内部好氧区与缺氧区比例的大小,还影响颗粒污泥内部物质的传质效果,进而影响到颗粒内部微生物的生存状态.有研究表明,好氧颗粒污泥具有微生物菌群的多样性,在降解有机碳的同时,具有硝化反硝化的功能[4,5].本研究采用气提式内循环间歇反应器(IC-SBAR)进行好氧颗粒污泥培养,并且对好氧颗粒污泥中具有脱氮功能的菌种特性进行了分析,旨在认识好氧颗粒污泥微观结构以及