低碳源浓度条件下聚磷菌特性-林海

�低碳源浓度条件下聚磷菌特性林�海�李�萍�陈月芳�王�倩�侯瑞荣�张�艳北京科技大学土木与环境工程学院,北京100083摘�要�从北京城市排水集团高碑店污水处理厂二沉池回流污泥中分离获得两株高效聚磷菌株,确定出稀释倒平板分离技术更有利于聚磷微生物的筛选.经形态特征及生理生化实验初步鉴定为不动杆菌属(acinetobactersp.).确定出A、E两菌的最优碳源选择分别为乙酸钠和谷氨酸钠;并在降低碳源质量浓度,即COD小于100mg�L-1的条件下进行最佳生长条件优化:A、E两菌的最适pH值分别为8和7,最适温度分别为20�和25�;金属镁、钾和锰离子对聚磷微生物具有积极促进作用.聚磷微生物呈现出较好的厌氧放磷、好氧摄磷的特性.关键词�聚磷菌(PAO);低碳源;筛选;生物除磷分类号�X703�1CharacteristicsofphosphorusaccumulatingorganismsatlowcarbonlevelLINHai,LIPing,CHENYue-fang,WANGQian,HOURui-rong,ZHANGYanSchoolofCivilandEnvironmentalEngineering,UniversityofScienceandTechnologyBeijing,Beijing100083,ChinaABSTRACT�Twoefficientpolyphosphateaccumulatingorganisms,AandE,werescreenedfromthereturnedsludgewhichcomesfromthefinalsedimentationtankinBeijingGaobeidianSewageTreatmentPlant.Atthesametime,thereversingdilutionplatesepa-rationtechnologywasidentifiedtobebeneficialtoscreeningthepolyphosphateaccumulatingorganisms.Observationsoftheirmorpho-logical,physiologicalandbiochemicalfeaturesindicatethattheyareacinetobactersp.Byexperiment,theoptimalchoicesforcarbonofthepolyphosphateaccumulatingorganismsAandEareCH3COONaandC5H8NNaO4,respectively.Experimentaldatashowthebestgrowthconditionsofpolyphosphateaccumulatingorganismsinreducingcarbonconcentrations,i.e.lessthanCOD100mg�L-1:theoptimalpHvaluesandtemperaturesofthepolyphosphateaccumulatingorganismsAandEare8and7,20�and25�,respec-tively.MetalionsMg2+,K+andMn2+canbehelpfultoaccumulatepolyphosphate.Theeffectofoxygenonthephosphateaccumu-latingmicroorganismswasdiscussed,andthecharacteristicsofPremovalinanaerobicconditionandPuptakeinaerobicconditioncouldbeobserved.KEYWORDS�phosphorusaccumulatingorganisms(PAO);lowcarbonsources;screening;biologicalphosphorusremoval收稿日期:2008-07-04基金项目:国家�十一五�科技支撑计划重大资助项目(No.2006BAC19B01)作者简介:林�海(1966�),男,教授,博士生导师,E-mail:linhai@ces.ustb.edu.cn��目前磷是影响水体富营养化的重要因素,对污水中磷的质量浓度进行有效的控制处理是十分必要的[1-2].生物除磷法主要利用聚磷菌聚磷作用,相比化学除磷,具有运行费用低,减少二次污染,减少活性污泥膨胀现象,是目前大多数污水处理厂所采用的除磷方法[3].最早从活性污泥中分离出的是不动杆菌属[4],迄今为止分离出的聚磷菌为数不多.大部分污水处理厂在除磷工艺处理中由于碳源含量过低即COD值较低,降低了聚磷微生物的除磷效率,并最