不同碳氮比值下亚硝酸盐氧化菌和异养菌混合体系的微生物多样性

应用与环境生物学报2009，15(3):351~355ChinJApplEnvironBiol=ISSN1006-687X2009-06-25DOI:10.3724/SP.J.1145.2009.00351Biologicalnitrificationprocesshasbeenwidelyappliedtoremovenitrogenpollutants(ammoniaandnitrite)inmunicipalwastewatertreatmentplants(WWTPs).Theprocessofnitrification,includingammonia-oxidizingprocessandnitrite-oxidizingprocess,wasperformedusingtwophylogeneticallyunrelatedgroupsofobligatelychemolithotrophicbacteria.Ammonia-oxidizingbacteria(AOB)firstoxidizeammoniatonitrite,andsubsequentlynitrite-oxidizingbacteria(NOB)oxidizenitritetonitrate.Variousparametersinfluencethenitrificationprocess.Majorfactorsoftheprocessincludedissolvedoxygen(DO),temperature,pH,ammoniaandnitriteconcentrations,organicloading,andhydraulicloadingrate[1].Theeffectofsolubleorganicmatteronnitrificationwasextensivelyinvestigatedbymanyresearchers[2~4].TheirresultsindicatedthathighC/Nratiosinducedinterspecificcompetitionforoxygenbetweennitrifyingbacteriaandheterotrophicbacteria,whichresultedinnitrificationretardance.However,sofartheeffectsoforganiccarbononnitrificationwerestudiedwithrespecteithertoreactorperformanceor不同C/N值下亚硝酸盐氧化菌和异养菌混合体系的微生物多样性*胡杰李大平**陶勇张金莲钟琦何晓红王晓梅(中国科学院成都生物所应用与环境微生物中心成都610041)MicrobialDiversityofNitrite-oxidizingandHeterotrophicBacterialCommunitiesunderDifferentC/NRatios*HUJie,LIDaping**,TAOYong,ZHANGJinlian,ZHONGQi,HEXiaohong&WANGXiaomei(CenterforAppliedandEnvironmentalMicrobiology,ChengduInstituteofBiology,ChineseAcademyofSciences,Chengdu610041,China)AbstractThemicrobialdiversityofnitrite-oxidizingandheterotrophicbacterialcommunitiesatthreeC/NratiosoffeedsolutionswasexperimentallyinvestigatedusingPCR-restrictionfragmentlengthpolymorphism(RFLP)techniqueandcorrelatedwithnitrificationperformancesofthemixture.AtC/N=0,thedominantpopulationsweremainlyautotrophicandoligotrophicbacteria(85.1%),includingnitrite-oxidizingbacteria(NOB),andsomestrainsofBacteroidetes,Alphaproteobacteria,Actinobacteria,andgreennonsulfurbacteria.AtC/N=0.44,autotrophicbacteriareduced,whileheterotrophicbacteria