生物除磷系统启动期聚磷菌的FISH原位分析与聚磷特性

生物除磷系统启动期聚磷菌的FISH原位分析与聚磷特性亢涵,王秀蘅3,李楠,任南琪(哈尔滨工业大学城市水资源与水环境国家重点实验室,哈尔滨150090)摘要:应用FISH对以乙酸钠为碳源的强化生物除磷(EBPR)SBR反应器启动期的微生物进行原位分析,考察除磷生态系统形成过程中聚磷菌种群结构、空间分布关系动态变化及其聚磷特性.结果表明,以异养菌为主的活性污泥经过厌氧Π好氧驯化后,聚磷菌大量富集,在全菌中的比例由1115%增加到40148%.启动过程中,生物系统内菌群竞争持续进行:首先,聚磷菌淘汰异养菌,历时5d;聚磷菌种群内选择过程历时19d;经过优势聚磷菌群的二次增长后,共计34d完成生物除磷系统的启动.富集过程中快速增殖的聚磷菌不能立刻行使除磷能力,要有一段“积累期”形成一定的PHA和poly2P储备.表现为污染物去除效率滞后于聚磷菌的增殖,经过4～8d的“积累期”后上升出现峰值.二次增长的优势聚磷菌群也经过“积累期”后才发挥作用.FISH图片显示,快速增殖期的聚磷菌菌体小,菌群结构松散.经过“积累期”之后,菌体不断增大,并开始紧密聚集形成致密的团状,此时反应器处理效率较高.关键词:生物除磷;聚磷菌;FISH;积累期中图分类号:X70311文献标识码:A文章编号:025023301(2009)0120080205收稿日期:2008201228;修订日期:2008203218基金项目:国家自然科学基金项目(50508011)作者简介:亢涵(1982～),女,博士研究生,主要研究方向为微生物生理生态学与分子生态学及生物脱氮除磷技术,E2mail:3172kh@1631com3通讯联系人,E2mail:xiuheng@hit.edu.cnCharacterizationofPhosphate2AccumulatingOrganismsinStarting2upEBPRbyFISHAnalysisKANGHan,WANGXiu2heng,LINan,RENNan2qi(StateKeyLaboratoryofUrbanWaterResourceandEnvironment,HarbinInstituteofTechnology,Harbin150090,China)Abstract:Enhancedbiologicalphosphorusremoval(EBPR)processwasoperatedinalaboratory2scalesequencingbatchreactor(SBR)forone2monthfedwithacetateasthecarbonsource.Thecharacteristicandthemicrobialpopulationstructureandspacedistributiondynamicsofphosphate2accumulatingorganisms(PAOs)ofstart2upperiodwereanalyzedbyfluorescentinsituhybridization(FISH).TherelationshipbetweenenrichmentofPAOsandphosphorusremovalwasdiscussed.PAOscouldbeenrichedbyrecirculationactivatedsludgecontainingheterotrophsthroughanaerobicaerobicconditions.PortionofPAOsinthesludgeincreasefrom1115%to40148%.Bacteriapopulationcompetitionlasted34days.ItstartedfromPAOsreplacingheterotrophswhichcost5daysthenfollowedby19daysintra2specificcompetitionofPAOs.Thelaststepwasre2increasingofPAOspredominance.Phosphorusuptakebytheenrichedmicrobialcommunitywasnotobservedimmediately.Anaccumulating2phasewasnecessaryforPHAandpoly2Pstorage.Alag2stageof428daysexistedwhentakingtheperformanceofthereactorintoconsideration.PhosphorusremovalbythepredominantPAOsthroughintra2specificcompetitionwasachievedafter