低温下A_2_O工艺污泥膨胀的微生物群落结构变化_王秀朵
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2020-02-20 07:45:55
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低温下A2/O工艺污泥膨胀的微生物群落结构变化王秀朵1,2,秦莉3,潘留明1,2,赵乐军1,2,陈赓妮4,冯志桐4,端正花4,5(1.天津市市政工程设计研究院,天津300051;2.天津市基础设施耐久性企业重点实验室,天津300051;3.农业部环境保护科研监测所,天津300191;4.天津理工大学环境科学与安全工程学院,天津300384;5.天津大学环境科学与工程学院,天津300072)摘要:采用高通量测序技术研究了A2/O工艺低温运行下污泥膨胀的机理及调控措施。结果表明,在21℃—19℃—12℃的降温过程中,活性污泥的SVI值显著升高、COD去除率相应降低,曝气池污泥中微生物群落结构也发生了显著改变。低温下,酸杆菌取代了不耐低温细菌的优势地位,丝状菌α变形菌纲和类丝状菌CandidatedivisionTM7大量繁殖,微生物群落结构变化是引发该反应器发生低温污泥膨胀的根本原因。低温对A2/O反应器中活性污泥性能的影响是可逆的,在采取合适的调控措施后,污泥性能可恢复到较好的水平。关键词:A2/O工艺;污泥膨胀;低温;微生物群落;丝状菌中图分类号:X703文献标识码:C文章编号:1000-4602(2017)13-0104-05基金项目:公益性行业(农业)科研专项(201403014);中国农业科学院创新工程项目(2015-cxgc-lyj)通信作者:端正花E-mail:duanzhenghua@mail.nankai.edu.cnMicrobialCommunityStructureChangeinanA2/OActivatedSludgeBulkingatLowTemperatureWANGXiu-duo1,2,QINLi3,PANLiu-ming1,2,ZHAOLe-jun1,2,CHENGeng-ni4,FENGZhi-tong4,DUANZheng-hua4,5(1.TianjinMunicipalEngineeringDesignandResearchInstitute,Tianjin300051,China;2.TianjinEnterpriseKeyLaboratoryofInfrastructureDurability,Tianjin300051,China;3.Agro-EnvironmentalProtectionInstituteofMinistryofAgriculture,Tianjin300191,China;4.SchoolofEnvironmentalScienceandSafetyEngineering,TianjinUniversityofTechnology,Tianjin300384,China;5.SchoolofEnvironmentScienceandEngineering,TianjinUniversity,Tianjin300072,China)Abstract:Themechanismsandcontrolmeasuresofactivatedsludgebulkinginananaerobic-an-oxic-oxic(A2/O)reactoratlowtemperaturewerestudied,usingthewaterqualityparameterdetectingandthehighthroughputsequencingtechnology.Resultsshowedthat,inthe21℃-19℃-12℃cool-ingprocess,thesludgevolumeindex(SVI)valuewassignificantlyincreasedandtheCODremovalratewasdecreasedcorrespondingly,andthemicrobialcommunitystructureintheaerationtankwassignifi-cantlychangedaswell.ThedominantpositionofbacteriathatlackofresistancetolowtemperatureswasreplacedbyAcidobacteria;meanwhile,AlphaproteobacteriaandCandidatedivisionTM7hadalsopropa-gatedsignificantly.Therefore,theactivatedsludgebulkinginthisreactorwasmainlyattributedtothechangesinthemicrobialcommunitystructureatlowtemperatures.Theeffectoflowtemperatureonthe
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