Low temperature MBBR nitrification Microbiome anal

Low temperature MBBR nitrification Microbiome analysisLowtemperatureMBBRnitrification:MicrobiomeanalysisBradleyYounga,RobertDelatollaa,*,KevinKennedya,EdithLaflammeb,AlainStintzicaDepartmentofCivilEngineering,FacultyofEngineering,UniversityofOttawa,Ottawa,CanadabVeoliaWaterTechnologies,Montreal,Quebec,CanadacOttawaInstituteofSystemsBiology,DepartmentofBiochemistry,MicrobiologyandImmunology,FacultyofMedicine,UniversityofOttawa,Ottawa,CanadaarticleinfoArticlehistory:Received19July2016Receivedinrevisedform27December2016Accepted29December2016Availableonline5January2017Keywords:NitrificationBiofilmMBBRMicrobiomeanalysisNextgenerationsequencingLowtemperatureabstractThisstudyaimstoinvestigatepostcarbonremovalmovingbedbiofilmreactor(MBBR)nitrificationthroughthetransitionfrom20�Cto1�Candduringthroughlongtermoperationat1�C.FourpilotnitrifyingMBBRreactorswereoperatedatvariousammonialoadingratestoelucidatethetemperatureeffectsonammoniaremovalrates,cellviabilityandbacterialcommunities.Thetransition