一种测定活性污泥中高等微生物活性的方法

第29卷第7期2009年7月环境科学学报ActaScientiaeCircumstantiaeVo.l29,No.7Ju.l,2009基金项目:国家自然科学基金项目(No.50678017);北京市属市管高等学校人才强教计划项目(No.BJE10016200611)SupportedbytheNationalNaturalScienceFoundationofChina(No.50678017)andtheFundingProjectforAcademicHumanResourcesDevelopmentinInstitutionsofHigherLearningundertheJurisdictionofBeijingMunicipality(No.BJE10016200611)作者简介:曹亚莉(1978)),女,讲师,E-mai:lcaoyal@ibucea.edu.cn;*通讯作者(责任作者),E-mai:lhaoxiaod@ibucea.edu.cnBiography:CAOYali(1978)),female,lecturer,E-mai:lcaoyal@ibucea.edu.cn;*Correspondingauthor,E-mai:lhaoxiaod@ibucea.edu.cn曹亚莉,郝晓地,王啟林,等.2009.一种测定活性污泥中高等微生物活性的方法[J].环境科学学报,29(7):1395-1399CaoYL,HaoXD,WangQL,etal.2009.Aneffectivemethodofmeasuringtheactivityofhighermicrooganismsinactivatedsludge[J].ActaScientiaeCircumstantiae,29(7):1395-1399一种测定活性污泥中高等微生物活性的方法曹亚莉,郝晓地*,王啟林,张向萍北京建筑工程学院可持续环境生物技术研发中心,北京100044收稿日期:2008-11-21录用日期:2009-05-14摘要:采用机械破碎方式灭活活性污泥中高等微生物,并通过测定破碎前后泥样耗氧速率(OUR)之差别间接计算高等微生物的活性作用.实验结果表明,以分散机破碎活性污泥,高等微生物均可被有效地破碎灭活,且再培养138h后不再恢复.与此同时,对酵母菌破碎实验显示,机械破碎并未对其数量、形态以及活性产生太大影响,这表明机械破碎亦不会对个体尺寸比酵母菌更小的细菌构成任何影响.为使破碎前后活性污泥絮凝体形态基本一致,将破碎后的泥样经离心处理,以尽可能使絮凝体恢复如初(以SVI衡量).通过比较破碎前后泥样的OUR并计算可知,高等微生物活性相对于活性污泥总活性约占12%~14%.关键词:活性污泥;絮凝体;细菌;高等微生物;活性;机械破碎;酵母菌;耗氧速率文章编号:0253-2468(2009)07-1395-05中图分类号:X172文献标识码:AAneffectivemethodofmeasuringtheactivityofhighermicroorganismsinactivatedsludgeCAOYal,iHAOXiaodi*,WANGQilin,ZHANGXiangpingTheResearch&DevelopmentCentreofSustainableEnvironmentalBiotechnology,BeijingUniversityofCivilEngineeringandArchitecture,Beijing100044Received21November2008;accepted14May2009Abstract:Amechanicaldisruptionmethodwasexperimentallyappliedtokillhighermicroorganismsinactivatedsludge;theactivityofhighermicroorganismscouldbeindirectlycalculatedonthebasisofthedifferenceofoxygenuptakeratios(OURs)beforeandafterdisruption.Theexperimentsindicatedthatadispersercouldeffectivelybreakupandkillhighermicroorganismsandthattheydidnotreviveafterenrichmentfor138h.Asacontro,lyeastswerealsodisruptedbythedisperser,toshowthattheirnumbers,morphologyandactivitywerenot