活性污泥法驯化筛选好氧反硝化菌的试验研究_张凤君

第26卷第11期2006年11月环境科学学报ActaScientiaeCircumstantiaeVo.l26,No.11Nov.,2006作者简介:张凤君(1957—),男,教授,E-mail:zhangfengjun@jlu.edu.cn,Tel:0431-8499792;*通讯作者(责任作者)Biography:ZNANGFengjun(1957—),male,professor,E-mail:zhangfengjun@jlu.edu.cn,Tel:0431-8499792;*Correspondingauthor张凤君,戴宁,李卿,等.2006.活性污泥法驯化筛选好氧反硝化菌的试验研究[J].环境科学学报,26(11):1804-1808ZhangFJ,DaiN,LiQ,etal.2006.StudyonacclimatingandfiltratingtheaerobicdenitrifyingbacteriafromActivatedsludge[J].ActaScientiaeCircumstantiae,26(11):1804-1808活性污泥法驯化筛选好氧反硝化菌的试验研究张凤君1,*,戴宁1,李卿1,马玖彤2,郭艳微11.吉林大学环境与资源学院,长春1300262.吉林大学化学学院,长春130026收稿日期:2006-01-04修回日期:2006-09-25录用日期:2006-09-28摘要:通过活性污泥法SBR反应器驯化富集好氧反硝化菌.对于在驯化期间出现的污泥膨胀、DM培养液的添加时间和污泥驯化效果都进行了相应的讨论.从完成驯化的污泥中筛选出20株具有较高脱氮效率的好氧反硝化细菌,并对其中的3株FW3、FW6和FR4进行了进一步的研究.结果表明,3株菌的硝酸盐去除率均在60%以上;当C/N为5、pH值为7(FW3和FR4)或8(FW6)、温度为30℃时,3株菌的亚硝酸盐积累较小;在此条件下,3株菌代谢产气中N2含量与大气相比,均高出10%左右.关键词:活性污泥法;SBR反应器;富集驯化;好氧反硝化菌文章编号:0253-2468(2006)11-1804-05中图分类号:X703文献标识码:AStudyonacclmiatingandfiltratingtheaerobicdenitrifyingbacteriafromactivatedsludgeZHANGFengjun1,*,DAINing1,LIQing1,MAJiutong2,GUOYanwei11.CollegeofEnvironmentandresources,JilinUniversity,Changchun1300262.CollegeofChemistry,JilinUniversity,Changchun130026Received4Janurary2006;receivedinrevisedform25September2006;accepted28September2006Abstract:ThemethodofacclimatingaerobicdenitrifyingbacteriaisstudiedfromactivatedsludgebySBR.Thephenomenaofsludgebulking,thetimeofaddingtheDMandtheresultsoftheacclimatedsludgearediscussedduringtheacclimatingaerobicdenitrifyingbacteria.Finally,20strainsaerobicdenitrifyingbacteria,whichhavehigherdenitrificationefficiency,areisolatedfromtheactivatedsludge,andthefurtherresearchforthe3strainsoutofthe20arecarriedou.tTheresultsshowthat,forallthe3strains,theremovalrateofnitrateishigherthan60%.WhenC/Nis5,thepHis7(FW3andFR4)or8(FW6)andthetemperatureis30℃,theaccumulationofNO-2-Nislow;thecontentofN2inthebottlecontainingbacteriais10%higherthanthatintheatmosphere.Keywords:activatedsludge;SBR;acclimating;aerobicdenitrifyingbacteria1引言(Introduction)近年