好氧颗粒污泥胞外多聚物的提取及成分分析

第1卷第4期环境工程学报Vol.1,No.42007年4月ChineseJournalofEnvironmentalEngineeringApr.2007好氧颗粒污泥胞外多聚物的提取及成分分析张丽丽姜理英方芳陈建孟(浙江工业大学生物与环境工程学院,杭州310032)摘要EPS是微生物聚集体的重要组成部分,提取和分析好氧颗粒污泥的EPS利于深入研究这一新兴微生物聚集体。采用加热、超声、高速离心和加碱等5种方法提取好氧颗粒污泥的EPS,并分析其主要成分。结果表明,匀浆预处理是提取颗粒污泥EPS首要步骤,超声和加热分别适合EPS定性与定量分析,在35W、超声4min的条件下,活性污泥与好氧颗粒污泥EPS提取液中TOC产量分别为10513mg/gVSS和9615mg/gVSS;加热法(80℃,60min)提取的EPS产量略高于超声法,对于活性污泥和好氧颗粒污泥,TOC含量分别为14216mg/gVSS和15312mg/gVSS;蛋白质是活性污泥和好氧颗粒污泥EPS中比例最大的成分,且蛋白与多糖在好氧颗粒污泥与其在絮状活性污泥中的比值范围分别为3122～5180和1168～2163。关键词好氧颗粒污泥胞外多聚物提取分析中图分类号X512文献标识码A文章编号167329108(2007)0420127204ExtractionandcompositionanalysisofEPSforaerobicgranularsludgeZhangLiliJiangLiyingFangFangChenJianmeng(SchoolofBiologicalandEnvironmentalEngieering,ZhejiangUniversityTechnology,Hangzhou310032)AbstractEPSwasthemaincomponentsofmicrobialaggregates,andtheextractionandanalysisofEPSforaerobicgranularsludgewasveryusefulforstudyingthisnovelmicrobialaggregate.Fivemethodsincludingheating,ultrasonication,high2speedcentrifigation,EDTA,andNaOHplusheatingwereemployedforextractingEPSofaerobicgranularsludge,andthekeycomponentsofEPSextractedwereanalyzed.Theexperimentalre2sultsrevealthatthehemogenizationwasanecessarystepforEPSextractionofgranularsludge.Throughcompari2son,theultrasonicationandheatingwerefitforqualitativeanalysisandquantitativemeasurement,respectively.Fortheultrasonication(35W,4min),theTOCinEPSextractionofactivatedsludgeandgranularsludgewas10513mg/gVSSand9615mg/gVSS,respectively.Forheating(80℃,60min),TOCproductionwas14216mg/gVSSand15312mg/gVSS,respectively.AnalysisforEPSextractedusingdifferentmethodsshowsthattheproteinswerethemostimportantcomponentsinthesludgesamples,andtheratiosofproteintopolysaccharidesingranularsludgeandflocsludgewere3122～5180and1168～2163,respectively.Keywordsaerobicgranularsludge;extracellularpolymericsubstances;extraction;analysis收稿日期:2006-06-09;修订日期:2006-09-15作者简介:张丽丽(1978～),女,讲师,博士,主要从事污水生物处理技术的研究。E2mail:misslyli0127@hotmail.com胞外多聚物(EPS)是分布于细胞表面的高分子有机物(Mw>10000),主要源于生物合成和分泌、细胞溶解及大分子水解等,通常认为它们有利于微生物细胞