一种从环境中分离和富集硝化细菌的方法

第31卷第6期2007年11月水产学报JOURNALOFFISHERIESOFCHINAVol.31Nov.N0.62()0丁文章编号:1000一O615(20()7)06一0737一06一种从环境中分离和富集硝化细菌的方法曾胡龙,蔡生力,戴习林,减维玲吃上海水产大学生命利一学与技术学院,l几海2()()()明)摘要:经反复分离筛选,从对虾养殖环境中分离得到5株亚硝化菌和3株硝化菌,均为革兰氏阴性细菌、设计了一套简易培养系统培养分离得到的硝化细菌,根据培养结果筛选出其中最具生长与硝化能力的优势亚硝化菌和硝化菌,同时检验应用该模式进行硝化细菌培养的效果L、培养结果表明筛选的几株硝化细菌均有很强的生长和硝化能力,其中Yl()5和X101分别是亚硝化菌和硝化菌中最具优势的菌株。采用所设计的简易培养模式,在遮光、30℃、连续无菌充气的培养条件下,同时不子{·加营养物质和调整pH值,即可在短时间内获得较高密度(10()一l了mL一’)的硝化细菌菌液,其中亚硝化菌富集培养时间为13一19d,石肖化菌为6一12d关键词:硝化细菌分离;富集培养;硝化能力中图分类号:5917;x172文献标识码:AAmethodforisolationandcultureofnitrobacteriafromenvironmentZENGHu一long,CAISheng一11,DAIXi一lin,ZANGWei一ling(曲11绍e‘了飞(lua一l必5〔·;e,*eea,;d几ehnol()g,,5人a,,ghu‘Fisher:e、之/,,i,少ersiz),sha鳍h“:20009‘),CI;‘,:“Abstraet:FivestrainsofnitrosobaeteriaandthreestrainsofnitrobacteriawereisolatedfromshrimPrearingenvironmentbyrePeatedseleetingonagarPurificationPlates.AlloftheisolationswereidentifiedasGram-negativebaeilluswithalengthofabout1.0一2.0卜m.AsimPleeulturesystemwasdesignedfortheeultivationofthesenitrifyingbacteria,whiehwasaimedtoseleetoutthemostPredominantstrains.TheeficieneyoftheeulturesystemwastestedsimultaneouslybyeValuatingthegrowthoftheselectednitrifyingbaeteriainit.TheeultureresultsshowthesestrainsselectedholdstrongPotentialityingrowthrateandnitrifieationsPeed.Amongthem,YIOS15thebestofnitrosobaeteriastrainsandX10115thebestofnitrobaeteriastrainsasfarasgrowthrateandnitrificationsPeedareconcerned.Furthermore,theresultshaveProvedthatthesimPleeulturesystem15eficientforcultivationofnitrifyingbaeteria.About13to19daysareneededfortheenriehmentofnitrosobaeteriawhilethatofnitrobaeteriarequires6to12days·PHadjustmentornutritionsuPPlyarenoteomPulsoryduringthewholeculturetermexeePtthebeginning.Are一arive一yhighdensity(about106一108mL一’)ofbacteriaeanbeobtainedwithconditionofappropriatetemperaturearound30℃,aerationanddarkenvironment.PHofthenitrosobaeteriaeulturemediadeereased收稿日期:2007刁3一2(-资助项目:上海市科技登山行动计划重点攻关项目}20()6(0639]9112)];上海科技兴农重叔攻关项日「沪农科攻字(2(j哪)第8一4号“虾类温室集约化健康养殖技术开发研究”」日海市重点学科建设项「1资助(Y1101)作者简介: