产嗅藻类对东太湖某地原水中嗅味物质2-MIB的贡献

中国环境科学2014，34(9)：2328-2333ChinaEnvironmentalScience产嗅藻类对东太湖某地原水中嗅味物质2-MIB的贡献邵晨，黎雷，于水利，侯立安，杨帆，殷祺。，漆晴(1同济大学环境科学与工程学院，上海200092；2．吴江华衍水务有限公司，苏州215220)摘要：针对东太湖某水源地原水中嗅味物质2-甲基异莰醇(2一MIB)~度过高问题，检测并鉴定原水中藻类分布及组成，考察并建立藻类与2-MIB的相关性，并利用16SrDNA基因测序方法对其中两种主要丝状藻类进行鉴定．结果表明：两个原水取水口及附近养殖区中2-MIB与藻密度呈明显的线性相关(>0．85)，且颤藻目浮丝藻、泽丝藻以及针杆硅藻等占总藻数量的55％N~．值得注意的是，存在于藻细胞内部的2-MIB比例可达68％，水处理过程中需考虑胞内2-MIB的释放风险．此外，对养殖区周围水质数据分析表明采样点中2-MIB浓度、藻密度、氨氮浓度均和其与离养殖区距离呈负相关性，表明该地区取水口附近的水产养殖可能是导致2-MB偏高的主要原因．关键词：2一甲基异莰醇；16SrDNA测序；藻源嗅味物质；胞内胞外；藻类代谢产物中图分类号：X524文献标识码：A文章编号：1000—6923(2014)09—2328—06Contributionofalgaeontheoccurrenceofodorandtastecompound2-MIBindrinkingwatersourceofEastTaiLake．SHAOChen，LILei，YUShui．1i，HOULi．an，YANGFan，YINQi，QIQingf1．CollegeofEnvironmentalScienceandEngineering，TongjiUniversity,Shanghai200092，China；2．HuayanWaterServiceCorporatiionlimited，Suzhou215220，China)．ChinaEnvironmentalScience，2014，34(9)：2328-2333Abstract：TofigureoutthereasonofsevereodorprobleminthewatersourceofEastTaihulake，thealgaespeciesandcelldensities．aswellastheircorrelationswith2一MIBconcentrationwereinvestigated．Twodominatedfilamentousalgaespeciesinthewaterwereidentifiedaccordingtol6SrDNAdetection．TheresultindicatedthaRhealgaecelldensityandtheconcentrationof2-MIBwereofhighco~elmionwithR>0．85．SometypicalodorcompoundsproducerOscillatoriales(includingtheidentifiedspeciesLimnothrixandPlanktothrix)andSynedraaccountforover55％ofthetotalalgaecellnumbers．Inparticular,theintraeellular2-MIBaccountfor68％ofitstotalconcentration,indicatingtheriskofintracellular2-MIBreleaseduringthewatertreatment．Inaddition，thedensityofalgaeaswellastheconcentrationof2-MIBandammonianitrogenweredecreasedwiththeincreasingdistancebetweensamplingpointsandtheaquiculturezone，suggestingthatthehighconcentrationof2·MIBaroundtheintakeswasprobablycausedbytheaquicultureactivities．Keywords：2-MIB；16SrDNAgeneidentification；algaerelatedodorandtastecompounds；intracellular-extracellular；algaemetabolismproducts嗅味问题是导致全世界范围饮用水质量遭投诉的最常见也是最主要的问题．欧美国家开展的相关研究较早，高达40％以上的水厂存在严重的嗅味问题⋯．我国