太湖缓冲带近自然湿地氨氧化细菌的筛选及降解效果比较

魏伟伟等太湖缓冲带近自然湿地氨氧化细菌的筛选及降解效果比较太湖缓冲带近自然湿地氨氧化细菌的筛选及降解效果比较*魏伟伟叶春李春华李定龙(1．常州大学环境与安全工程学院，江苏常州213100；2．中国环境科学研究院，环境基准与风险评估国家重点实验室，湖泊工程技术中心，北京100012)摘要以太湖缓冲带近自然湿地底泥为菌源，利用传统的富集、分离纯化等微生物手段，筛选出3株氨氧化细菌BW一1、BW～2、BW-3，检测其在富集培养液中的生长特征。测定3株细菌的16SrRNA基因序列，测序结果提交至GenBank进行同源性检索分析，并通过MEGA5．0软件进行比对和系统发育分析，结果显示菌株BW一1和BW一2皆为Nitrosomonassp．，BW-3为Nitrobactersp．。缓冲带近自然湿地中的NH4+一N属于低污染范畴，最高为2．11mg／L。以1o(体积分数)接种量接人约5mg／L的NH4+一N废水中培养12d，定量检测结果显示菌株BW-3去除效果最好，NHt—N去除率为73．86％，菌株BW-1对NH4+一N降解效果路差于BW-3，去除率为73．42，菌株BW-2对NHt—N降解效果最差，去除率仅为34．11。关键词氨氧化细菌湖泊缓冲带筛选鉴定Isolationofammoniaoxidizingbacteriafromnear-naturalwetlandinTaiLakebufferzonesanditsperformanceoilan]-moniadegradationWEJWeiwei1,2，YEChun，LIChunhua，LIDinglong．(1．SchoolofEnvironmentandS口Engineering，ChangzhouUniversity，ChangzhouJiangsu213100；2．StateKeyLaboratoryofEnvironmentalCriteriaandRiskAssessment，CentreofLakeEngineeringandTechnology，ChineseResearchAcademyofEnvironmentalSciences，Beijing100012)Abstract：Usingtraditionalmicrobiologymeansincludingenrichment，isolationandpurification，3autotrophicammonia-oxidizingbacteriaBW一1．BW一2andBW一3wereisolatedfromthesedimentofnear-naturalwetlandinTaiLakebufferzones．The3strainswereculturedinenrichmentbroth，andtheirgrowthcharacteristicswereinvestiga—ted．The16SrRNAgenesequencesof3strainsweredetectedandthensubmittedtoGenBankforhomologysearch，andphylogeneticanalysiswasperformedthroughMEGA5．0software．ResuhsshowedthatBW-1andBW一2werefi—nallydeterminedasNitrosomonassp．，andBW一3belongedtotheNitrobactersp．．Thenear-naturalwetlandinbufferzonesofTaiLakewasrankedas1owammoniapollutionareaswiththehighestammoniaconcentrationwas2。11mg／L．Inoculating10bacterialsuspensioninto5mg／Lofammoniawastewater，after12daysofculture，BW-3achievedthebestammoniaconversationrateof73．86％，thefollowingonewasBW一1，itsammoniaconversationratewas73．42，BW_1hadtheworstammoniaconversationrateof34．儿．Keywords：ammoniaoxidizingbacteria；lakebufferzones；separation；identification湖泊缓冲带是陆地与湖泊之间的缓冲区域，具有缓冲人类活动影响，截留、净化陆源污染物，改善湖泊水质的重要功能。太湖缓冲带内的近自然湿地，位于村落、农田和支浜之间，在截留转化农业非点源