土壤中有益放线菌的高效分离-筛选和生物测定技术

土壤中有益放线菌的高效分离-筛选和生物测定技术收稿日期:�2007�07�17基金项目:�浙江省重点项目(011102469)土壤中有益放线菌的高效分离、筛选和生物测定技术黄世文1,3,�高成伟2,�王�玲1,�王全永1,3(1.中国水稻研究所,杭州�310006;�2.广西师范大学,桂林�541004;�3.广西大学,南宁�530004)摘要�结合他人经验和近年作者的实际工作,本文系统介绍了土壤标本的采集、处理,放线菌的分离、纯化。放线菌分离物及其代谢产物对病原菌(真菌、细菌)、害虫和杂草的生物测定、评价,为研发微生物农药打下基础。关键词�土壤标本;�放线菌;�分离;�生物测定中图分类号�Q939.132High�performanceisolation,screeningandbioassaytechnologyforactinomycesfromsoilsamplesHuangShiwen1,3,�GaoChengwei2,�WangLing1,�WangQuanyong1,3(1.ChinaNationalRiceResearchInstitute,Hangzhou�310006,China;�2.GuangxiNormalUniversity,�Guilin�541004,China;3.GuangxiUniversity,Nanning�530004,China)Abstract�Thetechnologyandapproachesforsamplecollecting,samplehandling,isolationandpurificationofactino�mycesfromsoilsweresystematicallyintroducedbasedonthepreviousreportsandtheauthors�ownwork.Themethodsforbioassayandevaluationofactinomycesandtheirmetabolitestothepathogens(e.g.fungiandbacteria),insectpestsandweedswerealsointroduced.Keywords�soilsample;�actinomyces;�isolation;�bioassay��放线菌是产生抗生素活性物质最大