脱氮菌剂的构建及最优脱氮条件

2020/2/23脱氮菌剂的构建及最优脱氮条件https://x.cnki.net/read/article/xmlonline?filename=NCDG201703005&tablename=CJFDTOTAL&dbcode=CJFD&topic=&fileSourceType=1&taskId…1/11南昌大学学报(工科版),2017,03,231-235+240脱氮菌剂的构建及最优脱氮条件ConstructionofnitrogenremovalagentanditsoptimalnitrogenremovalconditionsWUMinOUYANGChaoyiLIULeiSchoolofResources,EnvironmentalandChemicalEngineering,NanchangUniversity吴敏欧阳超毅刘雷南昌大学资源环境与化工学院摘要:将实验室筛选得到的3株脱氮性能较好的异养硝化-好氧反硝化菌WN1、WN3以及DN02,通过混料设计考察各菌株的最佳复配比,以最佳复配比构建脱氮菌剂,并以碳源、pH、温度、ρCOD/ρTN以及转速为单因素探究菌剂的最优脱氮条件,验证菌剂在最优条件下的脱氮性能。结果表明:菌剂的最佳复配比φWN1=0.935%,φWN3=0.965%,φDN02=1.100%,在此复配比下,氨氮的去除率达到90.37%。在30℃,碳源为琥珀酸钠,pH为7,ρCOD/ρTN为12,转速为150r·min-1的菌剂最优脱氮条件下,菌剂的氨氮去除率为91.18%。关键词:异养硝化-好氧反硝化菌混料设计脱氮作者简介：吴敏(1992—),男,硕士生;刘雷(1964—),男,教授,lliu@ncu.edu.cn。收稿日期：2017-02-28Abstract：ThelaboratoryscreenedthreestrainsofWN1,WN3andDN02,whichwereheterotrophicnitrification-aerobicdenitrificationstrainsandhadgoodperformanceofnitrogenremoval.Theoptimummixtureratioofeachstrainwasinvestigatedusingmixturedesign.Inordertostudytheoptimumconditionsofnitrogenremoval,somefactorssuchascarbonsource,pH,temperature,ρCOD/ρTNandspeed,wereinvestigatedbytheexperimentofsinglefactor.TheresultsindicatedthattheoptimummixtureratioofφWN1was0.935%,φWN3was0.965%,andφDN02was1.100%,theremovalrateofammonianitrogenreached90.37%.Theoptimumconditionswereasfollows:thepHof7.0,thecarbonsourceofsodiumsuccinate,thetemperatureof30℃,theρCOD/ρTNof12andthespeedof150r·min-1.Underthiscondition,theremovalrateofammonianitrogenreached91.18%.Keyword：heterotrophicnitrification-aerobicdenitrificationstrains，mixturedesign，nitrogenremovalReceived：2017-02-28目前,我国已有超过66%的湖泊和水库存在不同程度的富营养化现象,其中重富营养和超富营养占到了22%[1]。未经处理或未达标的氨氮污、废水排放的增加是导致水体(特别是封闭水体)富营养化的重要原因[2],2020/2/23脱氮菌剂的构建及最优脱氮条件https://x.cnki.net/read/article/xmlonline?filename=NCDG201703005&tablename=CJFDTOTAL&dbcode=CJFD&topic=&fileSourceType=1&taskId…2/111材料与方法1.1材料1.1.1菌株来源1.1.2培养基1.1.3分析方法1.2方法1.2.13种菌株混合培养初探1.2.2混料设计确定菌剂复配比1.2.3菌剂最优脱氮条件研究由于这些含氨氮的污废水具有排放量大、毒性强、成分复杂、处理难度大的特点,使得氨氮污废水的处理研究成为国内外的热点[3]。传统的生