直接驯化嗜盐菌处理高盐废水的研究

宋昌等直接驯化嗜盐菌处理高盐废水的研究直接驯化嗜盐菌处理高盐废水的研究宋晶孙德栋王一娜薛文平董晓丽马春张新欣(大连工业大学化工与材料学院，辽宁大连116034)摘要从大连旅顺盐场底泥中筛选出适合高盐度的嗜盐菌，在序批式间歇反应器(SBR)中对其进行3．5％(质量分数)盐度的驯化，污泥混合液悬浮固体(MIsS)平均质量浓度达600mg／L。污泥比耗氧速率(SOUR)测量结果显示，内源呼吸阶段污泥SOUR为10．36mg／(g．h)，外源呼吸阶段污泥SOUR达到29．09mg／(g·h)，表明所筛选的嗜盐菌培养的污泥具有较高活性。利用培养的污泥进行高盐模拟废水处理试验，结果表明，对盐度为3．5％、COD为240～340mg／I。的高盐废水，在每周期12h、曝气量0．6L／min、污泥MLSS为600mg／L、污泥龄为18d条件下，COD去除率达95％以上。NHt-N去除率达61％，TP去除率达55％。改变进水有机负荷对出水COD去除影响不大，该系统耐有机负荷冲击能力较强；盐度负荷的改变对COD的去除影响不大，而NH才一N去除率有明显变化，在3．5％和5．0％的盐度下，NH，一N去除率分别为61％和31％．关键词嗜盐菌高盐废水SBRSOURAcclimatizationofhalophilicbacteriaforbiologytreatmentofhigh-saltwastewaterSongJing，SunDedong，WangYina，XueWenping，DongXiaoli，MaChun，ZhangXinxin．(CollegeofChemistryEngineering&Material，DalianPolytechnicUniversity，DalianLiaoningl16034)Abstract：ThehalophilicbacteriaadaptedtohighsalinitieswereisolatedfromthesubstratesludgeofDalianLvshunSaltFieldinChina．Thesebacteriawereacclimatedinthesequencingbatchreactors(SBR)withsalinitywas3．5％，andsludgewithaverageconcentrationof600mg／Lwasobtained．Thespecificoxygenuptakerate(SOUR)wasmeasured，andtheresultsshowedthattheendogenousandexogenousrespirationofoxygenuptakeratewerereached10．36mg02／(gVSS·h)and29．09mg02／(gVSS·h)respectively．Thisindicatedthatthesludgecultivat—edfromthehalophilicbacteriahadhighactivity．Asystemthatemployingthissludgewasbuiltforthetreatmentofhigh—saltwastewater，withtheoperatingconditionsofsalinityof3．5％，CODof240—340mg／L，anoperatingperiodof12h，aerationrateof0．6L／minandsludgeretentiontimeof18d，thesystemachievedtheCOD，NH4+一NandTPremovalefficiencyof95％，61％and55％respectively．TheorganicloadinghadlittleeffectonCODremoval，whichmeanstheresistancecapability,ofthissystemagainstorganicloadingwashigh．NH+一Nremovalefficiencyofthesys—ternwashighlycorrelatedwithsalinityloading，whenthesalinityloadingwas3．5％and5％，theremovalefficiencyofNH，一Nwere6l％and31％respectively．Keywords：halophilicbacteria；high-saltwastewater；SBR)SOUR高盐废水是指盐度(以NaCI计)至少为1．0％(质量分数，下同)的废水，主要包括含盐工业废水、含盐生活污水和其他含盐废水n]。这些废水中除了含有有机污染物外，还含有大量的无机盐，如Cl-、S0i一、Na