厌氧除磷种源的筛选与厌氧除磷条件的研究_郭夏丽

郭夏丽,郑平,梅玲玲.厌氧除磷种源的筛选与厌氧除磷条件的研究[J].环境科学学报,2005,25(2):238-241GUOXiali,ZHENGPing,MEILinging.Screeningforthephosphatereducerandstudyontheirdephosphorizationconditions[J].ActaScientiaeCircumstantiae,2005,25(2):238-241厌氧除磷种源的筛选与厌氧除磷条件的研究郭夏丽,郑平＊,梅玲玲浙江大学环境工程系,杭州310029摘要:通过分批培养,考察各接种物培养始末总磷浓度的变化,比较了牛粪、猪粪、鸡粪以及来自两个不同污水处理厂的厌氧污泥的除磷能力,试验证明猪粪的除磷效果最佳.在此基础上,将猪粪与两种厌氧污泥按质量比3∶1∶1混合作为接种物,研究了厌氧生物除磷的条件,结果表明葡萄糖和蛋白胨分别是厌氧生物除磷的良好碳源和氮源;适宜的初始pH6.5,适宜的培养温度35℃.在适宜的除磷条件下,培养至第4天,猪粪与厌氧污泥混合物对总磷的去除率达到最大值(21.31%).外加钼和其它微量元素对厌氧生物除磷没有效应,表明试验所用的培养液中,不缺少这些元素.另外,外加还原剂硫化物对厌氧生物除磷也没有明显的作用,表明培养液中的有机物质所致的低氧化还原电位已能满足厌氧生物除磷的要求.关键词:厌氧除磷种源;厌氧除磷条件文章编号:0253-2468(2005)02-0238-04中图分类号:X703文献标识码:AScreeningforthephosphatereducerandstudyontheirdephosphorizationconditionsGUOXiali,ZHENGPing,MEILingingDepartmentofEnvironmentalEngineering,ZhejiangUniversity,Hangzhou310029Abstract:Thebiologicalreductionforphosphatetophosphinecanbeusedtodevelopanewbiotechnologyforphosphateremovalfromwastewater.Asuitableinoculumshouldbepreparedandtheconditionsofthereactionshouldbeclear.BymeasuringthecontentsoftotalPbothatthebeginningandattheendofbatchcultivation,thephosphatereductionactivitiesofcowmanure,swinemanure,chickenmanureandtwokindsofanaerobicactivatedsludgefromdifferentWWTPwerecompared,andtheswinemanurewasfoundtobethebestoftheinocula.Basedonthat,theswinemanurewasmixedwithtwokindsofanaerobicactivatedsludgeatthemassratioof3∶1∶1,andthemixturewasthenusedastheseedingsludgetotesttheoptimalconditionsforphosphorusremovalinanaerobicsurroudings.Theresultsshowedthattheoptimalcarbonandnitrogensourceswereglucoseandpeptone,respectively.TheoptimalinitialpHvaluewas6.5andthetemperaturewas35℃.Undertheoptimalconditionsthemaximalphosphateremovalefficiencywas21.31%duringthefour-daycultivation.Noobviouseffectontheanaerobicphosphorusremovalwasfoundwhenmolybdenumandothertraceelementsaswellassulfideasareducingagentwereaddedintotheculturemedia.Keywords:phosphatereducer;dephosphorizationconditions收稿日期:2004-03-15;修订日期:2004-07-20基金项目:国家自然科学基金资助项目(批准号:50278084)作者简介:郭