铜绿微囊藻和蛋白核小球藻生长及光合活性对温度和光照交互作用的响应
- 海之魂
-
1 次阅读
-
0 次下载
-
2020-03-09 11:00:37
文档简介:
Sci.(湖泊科学),2014,26(5):773_779http://www.jlakes.org.E—mail:jlakes@niglas.ac.cn@2014byJournalofSciences铜绿微囊藻(Microcystisaeruginosa)和蛋白核小球藻(Chlorellapyrenoidosa)生长及光合活性对温度和光照交互作用的响应刘世明,朱雪竹H,张民(1:南京农业大学资源与环境科学学院,南京210095)(2:中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室,南京210008)摘要:通过室内纯培养,分别设定不同温度梯度(14、16、18%)和光照梯度(100、250、360p~mol/(m·s)),以模拟全球气候变化条件下,温带湖泊春季温度升高与光照减弱交互作用对铜绿微囊藻和蛋白核小球藻生长和光合活性的影响.通过流式细胞计数测定其生长曲线,观测其变化趋势,并利用叶绿素荧光仪PHYTO—PAM测定藻类光合活性.结果表明,当温度为18%,光强为100Ixmolf(m·s)时,铜绿微囊藻生长速率最大,细胞密度达到2.99X10cells/m1.在该温度和光照强度下,其光合活性也远高于其他实验组,其F/F值达到最大值0.39.蛋白核小球藻在温度为16和18℃时,其生长速率差异不大,但明显高于在温度为14%时的生长速率,其光合活性处于波动状态.与蛋白核小球藻相比,温度升高和光照减弱对铜绿微囊藻生长和光合活性的促进作用更为明显,而且随着温度的升高,铜绿微囊藻生长对低光的偏好更为显著.因此,全球变暖所导致的高温低光环境可能有利于确立铜绿微囊藻在湖泊中的优势地位.关键词:铜绿微囊藻;蛋白核小球藻;升温;光减弱;光合活性GrowthandphotosyntheticcapacityofMicrocystisaeruginosaandChlorellapyrenoidosainresponsetotheinteractionsoftemperatureandlightLIUShiming.ZHUXuezhu&ZHANGMin(1:CollegeofResourceandEnvironmentalSciences,NanjingAgriculturalUniversity,Nanjing210095,P.R.China)(2:StateKeyLaboratoryof妇ScienceandEnvironment,NanjingInstituteofGeographyandLimnology,ChineseAcademyofSciences,Nanjing210008,P.R.China)Abstract:Tosimulatetheefectoftheinteractionsofelevatedtemperatureandweakenedlightinspringonthegrowthandphoto—syntheticcapacityofMicrocystisaeruginosaandChlorellap~enoidosa,anexperimentwasperformedwiththesetwostrainsatdifferenttemperatures(14,16and18~(2)andlightintensities(100,250and360m0l/(m·s).Growthcurveswereobtainedbytheresultsofcellcountingwithflowcytometrytoreflecttheirgrowthtrends.PhotosyntheticcapacitywasmeasuredbyPhyt0-PAMfluorometry.Theresultsshowedthatthegrowthrateofaeruginosareacheditspeakatthecelldensityof2.99X10。cells/mlwhenthetemperatureWasat18%andlightintensitywas100moL/(m。s).Atthesametime,itsphotosyntheticcapacitywasmuchhigherthanothergroups,andFv/Fmvaluesreached0.39.WhilethegrowthratesofC.pyrenoidosaatthetemperaturesof16and18%weresimilar,theyweresignificantlyhigherthanthegrowthrateat14%,andthephotosyntheticcapacitykeptfluctuatingthroughouttheexperiment.O
评论
发表评论