光照强度和磷浓度对寡枝刚毛藻生长的影响

中国环境科学2015,35(7)：2153~2159ChinaEnvironmentalScience光照强度和磷浓度对寡枝刚毛藻生长的影响郭亮亮1,2,周维成1,2,周起超3,李根保1*(1.中国科学院水生生物研究所,淡水生态与生物技术重点实验室,湖北武汉430072；2.中国科学院大学,北京100049；3.云南省环境科学研究院,高原湖泊流域污染过程与管理云南省重点实验室,云南昆明650034)摘要：在利用沉水植被对湖泊进行生态修复过程中,当营养盐有所降低,水体透明度增加有利于沉水植物生长时,丝状绿藻——刚毛藻会大量生长,从而影响生态修复效果.为了研究光照强度和磷浓度对寡枝刚毛藻(Cladophoraoligoclona)生长的影响,本实验用含有不同磷浓度(0.005,0.02,0.1,0.6mg/L)的BBM培养基,在不同光照强度[10,30,50µmol/(m2·s)]下培养寡枝刚毛藻16d.结果发现,在光照强度为10µmol/(m2·s),初始P浓度0.1mg/L以上时,刚毛藻鲜重增加(约0.05g),其余处理条件下,刚毛藻鲜重均减少,其中光强为50µmol/(m2·s),初始磷浓度为0.005mg/L时,刚毛藻鲜重减少量最大(约0.49g).叶绿素荧光参数Fv/Fm值的变化表现为光强越大,Fv/Fm的值越低.在光强为10µmol/(m2·s)时,Fv/Fm值在0.75左右.光强为50µmol/(m2·s)时,Fv/Fm值仅为0.3.在相同光强下,不同磷浓度对Fv/Fm值变化无显著影响.相对于磷浓度对刚毛藻生长的影响,光强对刚毛藻的生长影响更大,且高光强不利于刚毛藻的生长.关键词：寡枝刚毛藻；光照强度；磷浓度；叶绿素活性中图分类号：X172文献标识码：A文章编号：1000-6923(2015)07-2153-07EffectsoflightintensityandphosphorusconcentrationonthegrowthofCladophoraoligoclona.GUOLiang-liang1,2,ZHOUWei-cheng1,2,ZHOUQi-chao3,LIGen-bao1(1.StateKeyLaboratoryofFreshwaterEcologyandBiotechnology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan430072,China；2.UniversityofChineseAcademyofSciences,Beijing100049,China；3.YunnanKeyLaboratoryofPollutionProcessandManagementofPlateauLake-watershed,YunnanInstituteofEnvironmentalScience,Kunming650034,China).ChinaEnvironmentalScience,2015,35(7)：2153~2159Abstract：Intheprocessofecologicalrestorationusingsubmergedvegetation,greenfilamentousalgaeCladophorawillbebloomingwithdecreasednutrientsandincreasedwatertransparency.TodeterminetheeffectsoflightintensityandphosphorusconcentrationongrowthofC.oligoclona,thegrowthandphotosyntheticactivityofC.oligoclonaculturedinBBMmediumatdifferentconcentrationsofphosphorus(0.005,0.02,0.1,0.6mg/L)andlightintensities[10,30,50µmol/(m2·s)]for16dayswasexamined.Theresultsshowedthatatthelightintensityof10µmol/(m2·s),thefreshweightofC.oligoclonaincreasedbyabout0.05gatthephosphorusconcentrationsof0.1mg/Lorabove.However,thefreshweightofC.oligoclonadecreasedunderotherconditions.Thegreatestfreshweightlosswasobserved(about0.49g)atthehighestlig