复合型微生物絮凝剂产生菌生长周期中参数变化_杜宝山

第45卷第1期吉林大学学报(理学版)Vo.l45No.12007年1月JOURNALOFJILINUNIVERSITY(SCIENCEEDITION)Jan2007复合型微生物絮凝剂产生菌生长周期中参数变化杜宝山1,2,张玉玲1,张兰英1,姚军3(1.吉林大学环境与资源学院,长春130061;2.通辽职业学院,内蒙古自治区通辽028000;3.长春市城建疏浚工程有限公司,长春130021)摘要:研究复合型微生物絮凝剂产生菌的生长曲线变化在不同时期的pH值、基质-葡萄糖、硫酸铵含量、絮凝活性、产量变化、细胞絮凝效果变化等.结果表明,在整个生长周期中,pH值基本不变;碳氮源变化显著,并存在相关性;进入稳定期后,60h时,絮凝活性达到最大值97.35%,此后,絮凝活性数值下降,但不明显,絮凝剂产量最大值为3.02g/L;细胞絮凝效果与细胞生长呈正相关性.鉴定结果表明,3种单菌分别为Brevibacteriummcbrellneri,Burkholderiaglumae,Cryptococcusalbidusvaraerius.关键词:复合型微生物;絮凝剂产生菌;生长周期;参数变化中图分类号:X172文献标识码:A文章编号:1671-5489(2007)01-0138-05ParameterChangeinGrowthCycleofCompoundBioflocculantProductBacteriumDUBao-shan1,2,ZHANGYu-ling1,ZHANGLan-ying1,YAOJun3(1.CollegeofEnvironmentandResources,JilinUniversity,Changchun130061,China;2.CareerCollegeinTongliao,Tongliao028000,InnerMongoliaAutonomousRegion,China;3.Dredging-projectCo.Ltd.,Changchun130021,China)Abstract:Thechangesinthecontentofbothsubstrate-glucoseandammoniumsulfate,pHandflocculationactivityandoutputofcompoundmicrobialflocculantindifferentperiodsofgrowthcyclecompoundbioflocculantproducing-bacteriumwerestudied.Inthewholegrowthcycle,pHwasstable,thechangeofcarbonandnitrogensourcewasobvious,thecontentofnitrogenwasconsistentwiththesourceofcarbon;themaximumofflocculation-activityreachedto97.35%at60hpointaftersteadyperiod,thenthenumericalvalueofflocculationactivityfelldown,butnotobvious.Flocculationactivityhadawideregionoflargestvalueandthemaximumoutputofflocculation(3.02g/L)wasinthisregion.Theresultofcellflocculationandgrowthofcellgotpositivepertinence.Appraisalresultis:3bacteriaarerespectivelyBrevibacteriummcbrellneri,BurkholderiaglumaeandCryptococcusalbidusvaraerius.Theexperimentalresultofferedafoundationtofurtherinvestigationsofcontributingfactoronflocculationandmechanism.Itprovidedgistintheoryforcultureinindustrialapplication,too.Keywords:compoundmicrobial;flocculationproduct-bacterium;growthcycle;changeofparameter收稿日期:2006-03-10.作者简介:杜宝山(1963～),男,汉族,硕士,副教授,从事环境化学的研究.联系人:张玉玲(1973～),女,蒙古族,博士,从事环境生物技术的研究,E-mall:lingling