微生物絮凝剂产生菌的选育及絮凝特性研究_费文砚

[22]KimMH,KongYJ,BaekH,etal.OptimizationofcultureconditionsandmediumcompositionfortheproductionofmicrococcinGO5byMicrococcussp.[J].J.Biotechnol,2006,121(1):54-61.[23]ZhaoJ,ZhuW,HuQ.EnhancedcatharanthineproductioninCatharan-thusroseuscellculturesbycombinedelicitortreatmentinshakeflasksandbiore-actors[J].EnzymeMicrobTechnol,2001,28(7-8):673-681.微生物絮凝剂产生菌的选育及絮凝特性研究费文砚,吴涓＊(安徽大学生命科学学院,安徽合肥230039)摘要:目的:从土壤中筛选具有良好絮凝活性的菌株进行优化培养并研究其絮凝特性。方法:采用平板划线法分离纯化获得目的菌株,通过单因素和正交实验确定最适培养、絮凝条件,考察该菌的生长及产絮凝剂的周期和絮凝活性分布等特征,及其对次甲基兰的脱色能力。结果:得到絮凝活性较高的菌株C5,其最适产絮条件为:初始pH值7.5,温度36℃,培养时间48h;最适培养基为:葡萄糖10g,KH2PO42g,K2HPO45g,(NH4)2SO40.2g,尿素0.5g,酵母膏0.5g,NaCl0.1g,MgSO4·7H2O0.2g,絮凝率可达92.0%;最适絮凝条件为:2.5mL发酵液,pH6.0,5mLCaCl2溶液;絮凝活性成分主要为多糖和蛋白质,多分布于胞外上清液中;发酵液的絮凝活性与细胞生长量均在培养48h时达最大;对次甲基兰的脱色能力优于硫酸铝和聚丙烯酰胺,2min内脱色率可达97%。结论:菌株C5可产高絮凝活性的絮凝剂,具有良好的应用前景。关键词:微生物絮凝剂;筛选;絮凝活性;絮凝率;脱色中图分类号:X172文献标识码:A文章编号:1004-311X(2007)03-0059-05ScreeningofBioflocculant-producingStrainandStudyonFlocculatingCharacteristicsFEIWen-yan,WUJuan＊(InstituteofLifeSciences,AnhuiUniversity,Hefei230039,China)Abstract:Objectives:Abioflocculant-producingstrainwithhighflocculatingactivitywasisolatedfromsoil,anditsflocculatingcharacteristicswereinvestigated.Methods:Thetargetstrainwasobtainedbyplatethreadseparatemethod,purifyandscreenpaths.Optimalculture-flocculat-ingconditionswerefoundbysinglefactandmultifactorsorthogonalexperiments.Thegrowthperiod,flocculant-producingperiodandactivitydistributionwereinvestigated.Thedecolorizationexperimentsofmethylenebluebybioflocculant,Al2(SO4)3andpolyacrylamidewerecarriedout,respectively.Results:Abioflocculant-producingstrainnamedC5withhighflocculatingactivitywasobtained.Theoptimumcultureconditionswere:pH7.5ofinitialpH,36℃ofculturetemperature,and48hofcultureperiod,andtheoptimumculturemediumwereglucose10g,KH2PO42g,K2HPO45g,(NH4)2SO40.2g,urea0.5g,yeast0.5g,NaCl0.1g,MgSO4·7H2O0.2g,and92.0%offlocculatingratewasreached.Throughorthogonaltest,theoptimalflocculatingconditionswerefoundtobe:2.5mLofmicrobialflocculantdosage,pH6.0an