焦化废水处理系统微生物群落结构动态的ERIC-PCR 指纹图谱分析

文章编号:025322468(2003)0620705206中图分类号:X703文献标识码:A焦化废水处理系统微生物群落结构动态的ERIC2PCR指纹图谱分析高平平1,2,赵义3,赵立平23(11山西大学生物技术研究所,太原030006;21上海交通大学生命科学技术学院,微生物分子生态学与基因组学实验室,上海200240;31太原理工大学环境工程系,太原030024)摘要:ERIC2PCR群落指纹图谱监测与常规技术相结合,分析了焦化废水处理系统曝气池的微生物群落结构动态变化与污染负荷和主要污染物降解效果变化的关系.在每3d1次,连续8个时间点的监测中,待处理废水中苯酚负荷在35153mgΠL和132182mgΠL之间波动,氰化物负荷从0196mgΠL变化到34175mgΠL,CODCr最低为1044110mgΠL,最高时为7930190mgΠL.第4监测期间高浓度矿物油冲击使活性污泥的沉降能力和对CODCr的降解效率显著降低,但对苯酚和氰化物的降解能力造成的影响较小.监测期间2个曝气池微生物群落的ERIC2PCR指纹图谱的平均相似性系数(Cs)分别为9411%和9616%,多样性指数在1177—2134和1160—2116之间,表明用ERIC2PCR检测出的该系统中的活性污泥这部分微生物种群的结构比较稳定,可能构成了苯酚和氰化物去除效率在监测期间相对稳定的基础.关键词:活性污泥;微生物群落结构;ERIC2PCRAnalysisofthemicrobialcommunityofactivatedsludgeindifferentaera2tionbasinswithinanindustrialphenolremediationsystembyERIC2PCRfingerprintingGAOPingping1,2,ZHAOYi3,ZHAOLiping2(11InstituteofBiotechnology,ShanxiUniversity,Taiyuan030006;21SchoolofLifeScienceandBiotechnology,LabofMolecularMicrobialEcologyandGenomics,ShanghaiJiaoTongUniversity,Shanghai200240;31D2epartmentofEnvironmentalEngineering,TaiyuanUniversityofTechnology,Taiyuan030024)Abstract:Theoccurrence,settleabilityandactivityofactivatedsludgeinfirst(TJ1)andsecond(TJ2)aerationbasininaABprocessbasedindustrialphenolremediationsystemwereanalyzedbyERIC2PCR(EnterobacterialRepetitiveIntergenicConsensus2PCR)fingerprintingcom2binedwithactivatedsludgecharacterization(SV30、MLSS、SVI),andmajorpollutants(phenol,cyanideandCODCr)measurements.Duringthemonitoringperiod(onesamplingeverythreedaysfor8continuoussamplings,M1—M8),theconcentrationsofprimarypollutantsintheinfluentfluctuatedfrom35153mgΠLto132182mgΠLforphenol,between0196mgΠLto34175mgΠLforcyanideandfrom1044110mgΠLto7930190mgΠLforCODCr1ThepresenceofapollutionshockcausedbymineraloilcontaminationoccurredatM4monitoringstageresultedinadramaticdecreaseinactivatedsludgesettleabilityandCODCrremovalefficiency.Theintroductionofthisloadingshockhadnosignificantim2pactonthesystem’sabilitytodegradephenolandcyanide.ERIC2PCRbandingpatternsofactivatedsludgesamplesat8stagesshowedameanCsv