!bacteria-diversity-in-a-Nonsaline-Alkaline-Environment-Heterotrophic-Aerobic-Ppopulations

10.1128/AEM.70.12.7378-7387.2004.2004,70(12):7378.DOI:Appl.Environ.Microbiol.IgorTiago,AnaPaulaChungandAntónioVeríssimoPopulationsEnvironment:HeterotrophicAerobicBacterialDiversityinaNonsalineAlkalinehttp://aem.asm.org/content/70/12/7378Updatedinformationandservicescanbefoundat:Theseinclude:REFERENCEShttp://aem.asm.org/content/70/12/7378#ref-list-1at:Thisarticlecites27articles,15ofwhichcanbeaccessedfreeCONTENTALERTSmore»articlescitethisarticle),Receive:RSSFeeds,eTOCs,freeemailalerts(whennewhttp://aem.asm.org/site/misc/reprints.xhtmlInformationaboutcommercialreprintorders:http://journals.asm.org/site/subscriptions/TosubscribetotoanotherASMJournalgoto:onNovember3,2011byNATIONALSCIENCELIBRARY,CAShttp://aem.asm.org/DownloadedfromAPPLIEDANDENVIRONMENTALMICROBIOLOGY,Dec.2004,p.7378–7387Vol.70,No.120099-2240/04/$08.00�0DOI:10.1128/AEM.70.12.7378–7387.2004Copyright©2004,AmericanSocietyforMicrobiology.AllRightsReserved.BacterialDiversityinaNonsalineAlkalineEnvironment:HeterotrophicAerobicPopulationsIgorTiago,AnaPaulaChung,andAnto´nioVerı´ssimo*DepartamentodeZoologiaandCentrodeNeurocieˆncias,UniversidadedeCoimbra,Coimbra,PortugalReceived23March2004/Accepted16August2004Heterotrophicpopulationswereisolatedandcharacterizedfromanalkalinegroundwaterenvironmentgeneratedbyactiveserpentinization,whichresultsinaCa(OH)2-enriched,extremelydilutedgroundwaterwithpH11.4.Onehundredeighty-fivestrainswereisolatedindifferentmediaatdifferentpHvaluesduringtwosamplingperiods.Toassessthedegreeofdiversitypresentintheenvironmentandtoselectrepresentativestrainsforfurthercharacterizationofthepopulations,wescreenedtheisolatesbyusingrandomamplifiedpolymorphicDNA-PCRprofilesandgroupedthembasedonsimilaritiesdeterminedbyfattyacidmethylesteranalysis.Phenotypiccharacterization,determinationsofG�Ccontent,phylogeneticanalysesbydirectse-quencingof16SrRNAgenes,anddeterminationsofpHtolerancewereperformedwiththeselectedisolates.Although38differentpopulationswereidentifiedandcharacterized,thevastmajorityoftheisolat