养殖废水处理系统中微生物菌群结构及动态变化

中国环境科学2019,39(2)：839~848ChinaEnvironmentalScience养殖废水处理系统中微生物菌群结构及动态变化黄薇1,刘兰英1,吴妙鸿1,陈丽华1,吕新1,叶美锋2,林代炎2,宋永康1*(1.福建省农业科学院农业质量标准与检测技术研究所,福建福州350003；2.福建省农业科学院农业工程技术研究所,福建福州350003)摘要：采用高通量测序技术对养猪场废水处理系统的细菌群落组成进行分析,同时对细菌群落、污水水质和抗生素含量相关性进行分析.研究结果表明,污水各处理单元中的菌群结构存在显著差异,且细菌菌群结构随处理进程逐步改变,其中集污池与厌氧反应器的细菌菌群优势门类为Firmicutes、Bacteroidetes和Proteobacteria,曝气池的细菌菌群优势门类变为Firmicutes、Verrucomicrobia、Bacteroidetes、Proteobacteria和CandidatusSaccharibacteria,氧化塘的细菌菌群优势门类为Firmicutes、Proteobacteria、Chloroflexi、Euryarchaeota和Bacteroidetes.相关性分析结果表明,污水中的细菌群落与水质环境因子之间存在显著相关(P<0.05).其中,种属Acinetobacter、Bacteroides、Prevotella、Lachnospiracea_incertae_sedis与COD、TN、NH4+-N呈显著正相关,Clostridiumsensustrict、Methanothrix与TN、NH4+-N呈显著负相关,Petrimonas、Lachnospiracea_incertae_sedis、ClostridiumXlVa、Tissierella与磺胺甲恶唑、磺胺间甲氧嘧啶、强力霉素、土霉素呈极显著正相关,Acinetobacter、Bacteroides、Prevotella与恩诺沙星、四环素呈显著正相关,Prevotella、Lachnospiracea_incertae_sedis与环丙沙星呈显著正相关,Clostridiumsensustricto、Petrimonas与氧氟沙星呈显著正相关.关键词：养猪废水；细菌群落；水质；抗生素中图分类号：X172文献标识码：A文章编号：1000-6923(2019)02-0839-10Microbialcommunitystructureanddynamicsinswinewastewatertreatmentsystem.HUANGWei1,LIULan-ying1,WUMiao-hong1,CHENLi-hua1,LÜXin1,YEMei-feng2,LINDai-yan2,SONGYong-kang1*(1.InstituteofAgriculturalQualityStandardsandTestingTechnologyResearch,FujianAcademyofAgriculturalSciences,Fuzhou350003,China；2.InstituteofAgriculturalEngineeringandTechnology,FujianAcademyofAgriculturalSciences,Fuzhou350003,China).ChinaEnvironmentalScience,2019,39(2)：839~848Abstract：Toelucidatethebacterialcommunitydynamicsinswinewastewatertreatmentsystem,thebacterialcommunitycharacteristicsindifferenttreatmentunitsofswinewastewaterwasanalyzedbyIlluminaMiseqhigh-throughputsequencingtechnologies,andthecorrelationbetweenbacterialcommunityandwaterqualityorantibioticswasinvestigated.Theresultsdemonstratedthatmicrobialcommunitystructurevariesgreatlyindifferentwastewatertreatmentprocesses.ThedominantbacteriainfoulingpoolandanaerobicreatorwereFirmicutes,BacteroidetesandProteobacteria,thedominantbacteriainaeratio