Fe0对污泥接种餐厨垃圾厌氧发酵及抗生素抗性基因的影响
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2020-03-19 14:27:01
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第37卷第7期2019年7月环境工程EnvironmentalEngineeringV01.37No.7Jul.2019Feo对污泥接种餐厨垃圾厌氧发酵及抗生素抗性基因的影响王攀1杜晓璐1陈锡腾1孙淑婷1梁肖飞1任连海1吕峥2(1.北京工商大学食品学院,北京100048;2.国家发展与改革委员会资源节约和环境保护局,北京100824)摘要:在中温条件下以污泥为接种物,以餐厨垃圾为发酵底物,通过向厌氧发酵系统中投加不同量的Feo,考察其对餐厨垃圾厌氧发酵过程中日产气量、累积产气量、SCOD、氨氮、挥发性脂肪酸(VFAs)以及厌氧发酵体系中抗生素抗性基因行为特征的影响(包括口一内酰胺类blaOXA-l、blaTEM,大环内脂类ermB、ermF、mefA,磺胺类sull、sul2,四环素类tetM、tetO、tetQ、tetW、tetX和I类整合子intll)。结果表明:投加Feo可以起到提高产气量,促进有机物的水解,缓解酸抑制的作用,当投加20g/LFeo时产气量最高。由于经过湿热水解预处理,原始餐厨垃圾的抗性基因丰度较低,但接种污泥中ermF、tetO、tetQ和tetW的丰度较高,分别为5.13×105,3.04x105,2.03x105,1.11×105copies/g,经厌氧发酵后这4种抗性基因丰度削减明显,但是mefa和tetM的丰度与原始投加物料相比有所增长,投加20g/LFeo与未投加Fe。相比,m彬、tetM2种抗性基因的丰度有一定削减。关键词:餐厨垃圾;厌氧发酵;抗生素抗性基因;零价铁(Feo)DOI:10.13205/i.hjgc.201907032EFFECTSOFFeoONBIoGASPRoDUCTIoNANDGENESINANAERoBICDIGESTIoNoFFoODTHEFATEoFANTIBIoTICRESISTANCEWASTEINoCULATEDWITHSLUDGEWANGPanl,DUXiao—lul,CHENXi—ten91,SUNShu.tin91,LIANGXiao—feil,RENLian.hail,LVZhen92(1.SchoolofFoodandChemicalEngineering,BeijingTechnologyandBusinessUniversity,Beijing100048,China;2.DepartmentofResourceConservationandEnvironmentalProtection,NationalDevelopmentandReformCommission,Beijing100824,China)Abstract:Theanaerobicdigestionoffoodwastewasconductedundermesophilicconditionsinoculatedwithwastewatertreatmentsludge.TheinfluenceofFe”ontheanaerobicfermentationperformance,suchasgasproduction,SCOD,ammonia—nitrogen,volatilefattyacids(VFAs)andthefateofantibioticresistancegenes(ARGs),wasinvestigated.TheARGsinclude8.1actamsARGs(blaOXA一1,blaTEM),macrolidesARGs(ermB,ermF,m明),sulfonamidesARGs(sull,sul2),tetracyclineARGs(tetM,tetO,tetQ,tetW,tetX)andclassIintegronintll.TheresultsshowedthattheFe”couldincreasethegasproduction,promotethehydrolysisoforganicsandalleviatetheacidinhibition.When20g/LFe”wasaddedinthesystem,thegasproductionreachedthemaximumvalue.TheabundanceofARGsintheoriginalfoodwastewaslowduetothehydrothermalpretrealmenl,buttheabundancesofermF,tetO,tetQandtetWinthesludgewerehigher,detectedas5.13×105,3.04x105,2.03x105and1.11X105copies/g,respectively.TheabundanceofthesefourARG
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