聚糖菌颗粒污泥基于胞内储存物质的同步硝化反硝化_王景峰

聚糖菌颗粒污泥基于胞内储存物质的同步硝化反硝化王景峰1,2,王暄1,季民1＊,刘卫华1,杨造燕1(1.天津大学环境科学与工程学院,天津300072;2.军事医学科学院卫生学环境医学研究所,天津300050)摘要:采用特殊运行方式的厌氧-好氧SBR系统(厌氧后排水),以乙酸钠为有机基质成功富集了聚糖菌颗粒污泥.聚糖菌颗粒污泥厌氧-好氧批式实验表明,聚糖菌颗粒污泥具有较强的SND能力,TOC/N分别为5.0,4.0,2.8时,SND效率分别96.4%、95.3%及96.2%,而周期总氮去除效率随着碳氮比降低而降低,分别为66.0%、61.2%及56.3%.通过对周期氨氮、亚硝酸盐氮、硝酸盐氮、TOC以及胞内糖原、PHB变化的测定分析,证明聚糖菌颗粒污泥SND过程中,污泥以厌氧阶段储存于胞内的多聚物PHB作为反硝化碳源,并且反硝化聚糖菌是系统中反硝化能力的来源.与溶解性基质相比,PHB的降解速率相对较低,因此在SND过程中,反硝化可以与硝化保持相近的速率,从而有助于获得良好的SND效果.关键词:聚糖菌;反硝化聚糖菌;好氧颗粒污泥;胞内储存物质;同步硝化反硝化中图分类号:X703.1文献标识码:A文章编号:0250-3301(2006)03-0473-05收稿日期:2005-04-23;修订日期:2005-07-11基金项目:教育部天津大学-南开大学合作项目作者简介:王景峰(1974～),男,博士研究生.主要研究方向为污水生物处理理论与技术.＊通讯联系人IntracellularStoragePolymerDrivenSimultaneousNitrificationandDenitrificationofGAOsGranularSludgeWANGJing-feng1,2,WANGXuan1,JIMin1,LIUWei-hua1,YANGZao-yan1(1.SchoolofEnvironmentalScienceandEngineering,TianjinUniversity,Tianjin300072,China;2.InstituteofHygieneandEnvironmentalMedicine,AcademyofMilitaryMedicalSciences,Tianjin300050,China)Abstract:Glycogenaccumulatingorganisms(GAOs)granularsludgewascultivatedandstablyexistedinasequencingbatchreactor(SBR),fedwithacetateasorganicsubstrate,bymeansofspecialalternatinganaerobic-aerobicoperationmode,i.e.withdrawalafteranaerobicperiodandsludgeaerationduringaerobicperiodtosuppresstheactivityofphosphateaccumulatingorganisms(PAOs).Anaerobic-aerobicbatchtestsofGAOsgranularsludgewerecarriedouttoinvestigateitssimultaneousnitrificationanddenitrification(SND)performancesunderdifferentTOC/N.TheresultsshowedGAOsgranularsludgehadhighSNDactivity,SNDefficiencieswere96.4%,95.3%and96.2%respectivelyasTOC/Nwere5.0,4.0and2.8,andthetotalnitrogenremovalefficiencies,decreasedwithTOC/Ndecreased,were66.0%,61.2%and56.3%respectively.Measuringandanalyzingthevariationofammonia,nitrite,nitrate,TOC,intracellularglycogenandPHBinbatchtests,itwasverifiedthatintracellularPHBwasusedascarbonsourceofdenitrificationanddenitrifyingglycogenaccumulatingorganisms(DGAOs)wereresponsibleorganismsforthedenitrificationactivity.ThedegradationrateofPHBwa