溶藻细菌胞外活性物质对蛋白核小球藻的毒性效应

微生物学通报JUN20,2009,36(6):821~825Microbiology©2009byInstituteofMicrobiology,CAStongbao@im.ac.cn*通讯作者:Tel:86-20-39380556;:ppwbpan@scut.edu.cn收稿日期:2008-10-23;接受日期:2009-03-05研究报告溶藻细菌胞外活性物质对蛋白核小球藻的毒性效应张纯敏潘伟斌*陈岩贽(华南理工大学广东广州510006)摘要:为了探索分离到的溶藻细菌L7胞外活性物质对蛋白核小球藻的毒性效应和致毒机理,采用不同质量浓度的L7胞外活性物质冻干粉(L7-LPEAC)处理蛋白核小球藻(Chlorellapyrenoi-dosa),测定藻细胞的光合作用效率(EPR)以及蛋白质、叶绿素a和丙二醛(MDA)的含量。L7-LPEAC溶液浓度较低(0.80g/L和1.25g/L)时促进蛋白核小球藻的生长,其96h、120h的EC50分别为5.75g/L和2.55g/L。当L7-LPEAC溶液浓度≥2.00g/L时,叶绿素a和蛋白质含量变化同步呈现先增加后减少的趋势;处理72h后,L7-LPEAC溶液浓度分别为2.00g/L,3.13g/L,4.90g/L的浓度组中,藻细胞MDA含量与对照组相比差异显著(P<0.05),浓度组7.67g/L和12.00g/L则与对照组差异极显著(P<0.01);处理120h后,各浓度组藻细胞叶绿素a含量的相对抑制率均大于60%。使用L7-LPEAC修复富营养化水体时,选择适当的投加浓度,既能杀灭引起水体富营养化的目标藻类,又能避免对其他藻类产生抑制作用,可以较好地维持水生生态系统的平衡。关键词:蛋白核小球藻,毒理,溶藻细菌,溶藻活性物质TheToxicEffectsoftheExtracellularActiveComponentsfromOneAlgae-lysingBacteriaonChlorellaPyrenoidosaZHANGChun-MinPANWei-Bin*CHENYan-Zhi(CollegeofEnvironmentalScienceandEngineering,SouthChinaUniversityofTechnology,Guangzhou,Guangdong510006,China)Abstract:ToxiceffectsofL7lyophilizedpowderofextracellularactivecomponents(L7-LPEAC),extractedfromtheAlgae-lysingbacteriaL7,onChlorellapyrenoidosawerestudiedaccordingtothechangesofeffec-tivephotosynthesisrate(EPR),theproteincontent,thechlorophyllacontentandtheMDAcontentofalgae.TheresultsshowedthatthegrowthofalgawaspromotedatlowconcentrationsofL7-LPEAC(0.80g/L,1.25g/L).The96h-EC50and120h-EC50uponChlorellapyrenoidosaare5.75g/Land2.55g/L,respec-tively.ThechlorophyllacontentincreasedfirstlyandthendecreasedathighconcentrationsofL7-LPEAC(≥2g/L),sodidtheproteincontent.Comparedwiththecontrolgroup,thereisasignificantstatisticsdiff-erence(P<0.05)inthetreatmentgroups(2.00g/L,3.13g/L,4.90g/L),whileextremesignificantstatisticsdifference(P<0.01)intheothertreatmentgroups(7.67g/L,12.00g/L)after72h.TheinhibitionratesofchlorophyllacontentofChlorellapyrenoidosainalltreatmentgroupsreachedover60%after120h.Forthe822微生物学通报2009,Vol.36,No.6http://journals.im.ac.cn/wswxtbcnremediationofeutrophicnaturewaterbody,itcannotonl