分光光度法测定小球藻数量的方法研究

安徽农业科学，JournalofAnhuiA一．Sei．2009。37(23)：11104—11105责任编辑罗芸责任校对卢瑶分光光度法测定小球藻数量的方法研究吕旭阳，张雯，杨阳，蔡，J、宁+(南京晓庄学院生命科学系，江苏南京211171)摘要[目的]确定用分光光度法测定小球藻藻液浓度的适宜波长。[方法]在不同波长下对不同浓度普通小球藻藻液的吸光度进行测定，对有关数据进行生物统计分析。[结果】在8．7×10。～8．7×105个／“的浓度范围内，小球藻藻液的最大吸收波长在680nm左右，较高浓度小球藻藻液的吸光度超出了测定范围。显著性测验表明，9种波长下小球藻藻液浓度与藻液吸光度呈极显著直线相关。680nnl波长下的测定灵敏度最高，其最高藻液浓度最低，为3．79×108个／ml；700nm波长下的测定灵敏度最低，其最高藻液浓度最高，为7．7×108个／ml；560am波长下的测定灵敏度仅高于700nm波长下的。其最高藻液浓度为6．9×108个／ml：540nm波长下的测定灵敏度高于560珊波长下的，其最高藻液浓度为6．5×108+／ml。[结论]在680nm波长下测定小球藻藻液浓度，其灵敏度高、数据更精确，关键词普通小球藻；吸光度；相关系数中图分类号0657．32文献标识码A文章编号0517—661l(2009)23一11104—02MethodologicalResearchonMeasuringChloreHaQuantitybySpectrophotometryLVXu·yangetal(DepartmentofLifeScience，NanjingXiaozhuangCollege，Nanjing，Jiangsu211171)Abstract【Objective111eresearchaimedtoascertainthewavelengthsuitablefordeterminingtheeoncn．ofchlorellasuspensionbyspectro．photome”y．1Method】Theabsorbanceofthesuspensionwithdifierentconch．ofChlorellavulgarisweredeterminedatdifferentwavelengthsandthebiologicalstatisticanalysiswasperformedontherelevantdata．JResultIntheeoncfl．rangeof8．7×100一8．7×108／m1．themaxi．mumadsorptionwavelengthofchlorellasuspensionwasabout680nmandtheabsorbanceofchlorellasuspensionwithhigherconcn．exceededthedeterminationrange．Thesignificancetestshowedthattheeoncll．ofchlorellasuspensionshowedextremelysignificantlinearcorrelationwithitsabsorbanteat9wavelengths．Thedeterminationsensitivityatthewavelengthof680nmwashighestanditshighestsuspensionconcn．waslowest．being3．79×105／m1．Thedeterminationsensitivityalthewavelengthof700nmwasIowestanditshighestsuspensionconcn．washighest，being7．7×105／m1．Thedeterminationsensitivityatthewavelengthof560amwasiusthigherthanthatatthewavelengthof700nlnanditshighestsuspensioncOnCH．was6．9×105／m1．ThedeterminationsensitivityatthewaveleElgthof540nmwashigherthanthatatthewavelengthof560nmanditshighestsuspensionconcn．was6．5X106／m1．IConclusion】耵IedeterminationontIleconch．ofchlorellaSUS．pensionatthewavelengthof680nmhadhighsensitivityandmoreprecisedata．KeywordsChlorellavulga