不同DO下MBR内微生物群落结构与运行效果关系

中国环境科学2010，3O(2)：209-215ChinaEnvironmentalScience不同DO下MBR内微生物群落结构与运行效果关系高大文，李听芯，安瑞，付源，任南琪(哈尔滨工业大学城市水资源与水环境国家重点实验室，黑龙江哈尔滨150090)摘要：应用A／O—MBR处理实际生活污水，考察了不同溶解氧(DO)条件下，微生物群落结构与其处理效果的对应关系．结果表明，DO浓度在0．2-4．0mg／L对COD去除效果无明显影响，COD平均去除率均在90％以上．DO浓度变化对NI-h一N去除影响较大，DO浓度下降到0．2mg／L时，N一一N平均去除率由99％T降到65％．通过PCRDGGE分析，较高DO条件T(4．0，2．Omg几)的总细菌微生物群落多样性高于较低DO条件fO．5，o．2mg／L)，但其群落结构变化与与反应器的处理效果对应关系不明显；氨氧化菌的群落结构变化较明显，且在不同的DO条件下起主要作用的氨氧化菌的菌属不同，其群落变化与反应器的NH4+-N去除效果相对应，DO为2．0，0．5metE时氨氧化菌群落结构比较相似，此时反应系统的脱氮效果也比较好．关键词：膜生物反应器；溶解氧：PCR-DGGE；微生物群落结构：氨氧化菌中图分类号：X703．1文献标识码：A文章编号：1000—6923(2010)02—0209—07RelationshipsbetweenmicrobialcommunitystructureandtheperformanceofMBRunderdifferentdissolvedoxygen．GAODa．wen，LIXin．xin，ANRui，FUYuan，RENNan-qi(1：StateKeyLaboratoryofUrbanWaterResourceandEnvironment，HarbinInstituteofTechnology,Harbin150090，China)．ChinaEnvironmentalScience，201O，3O(2)：209-215Abstract：Microbialcommunitystructureandnutrientremovalperformanceunderdifferentdissolvedoxygenwereinvestigatedusingmnoxic—oxicsubmergedmembranebioreactor(A／O-MBR)formunicipalwastewatertreatment．ResultsshowedthattheCODremovalwasnotinfluencedmarkedlybythevariationsinDObetween0．2mg／Land4．0mg／L．andtheCODremovalWasmorethan90％duringthewholeexperiment．However，theammonianitrogenremovalwasmoresensitivethanCOD．andtheammonianitrogenremovaldroppedfrom99％to65％whenDOdecreasedto0．2mg／L．ThePCR-DGGEanalysisshowedthatthediversityoftotalbacterialcommunityWasabundantwhenA／O-MBRwaslUllathighDO(4．0mg／Land2．0mg／L)，andtherelationshipsbetweenthetotalbacterialcommunitystructureandtheperformanceofMBRWasnotveryobvious．Onthecontrary，theammoniaoxidizingbacterialcommunitycompositionhadamoreobviousshiftwiththevariationofDOconcentration，andthespeciesofammoniaoxidizingbacteriawhichplaymajorrolewerediferentunderdifferentDOconditions，whichwerecorrespondedtotheremovaleficiency．Theamr~oniaoxidizingbacterialcomunitycompositionweresimilarundertheDOconcentrationof2．0mg／LandO．5mg／L，andthenitrogenrenmovalWasenhanced．Keywords：membranebioreactor(MaR1：dissolvedoxy