MBR+蠕虫反应器膜污染特征及微生物群落结构
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2020-04-07 18:12:46
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第49卷第2期2017年2月哈尔滨工业大学学报JOURNALOFHARBININSTITUTEOFTECHNOLOGYVo1.49No.2Feb.2017DOI:10.11918/j.issn.0367-6234.2017.02.006MBR+蠕虫反应器膜污染特征及微生物群落结构刘嘉,左薇,张军,李慧,李俐频,田禹,(1.城市水资源与水环境国家重点实验室(哈尔滨工业大学),哈尔滨150090;2.哈尔滨工业大学市政环境工程学院,哈尔滨150090)摘要:为研究针对MBR+蠕虫反应器工艺中蠕虫捕食对膜污染的影响,在常温下分别平行运行MBR+蠕虫反应器(R1)和作为对照系统的MBR+空白蠕虫反应器(n2).监测R1工艺中MBR(S-MBR)和R2工艺中MBR(C-MBR)的跨膜压力(P),检测污泥混合液及泥饼层微生物代谢产物的变化.利用变性梯度凝胶电泳(DGGE)技术分析S-MBR和C—MBR中微生物种类和分布.结果表明:S-MBR的膜污染周期为90d,C-MBR的膜污染周期为28—37d,蠕虫捕食导致S-MBR中SMP和EPS的多糖和蛋白质减少.S—MBR膜丝表面是微生物茵群Alphaproteobacterium,Betaproteobacterium,Deltaproteobacterium和Geobacter,而C—MBR膜丝表面是微生物菌群Azorhizobium,Rhodobacter,Gammaproteobacterium和Flavobacteria,对MBR膜污染进程起主要作用.Caldilinea可能与S-MBR膜污染减轻有关.蠕虫捕食可改变微生物群落结构,减缓S-MBR膜污染.关键词:膜生物反应器;膜污染;胞外聚合物;溶解性微生物代谢产物;微生物群落结构中图分类号:X172文献标志码:A文章编号:0367—6234(2017)02-0032-05Amaysisofinmtra~foulingandnicroi~conmntyscmlctu~inallMBR+~mmreactorLIUJia,ZUOWei,ZHANGJun,LIHui,LILipin,TIANYu'(1.StateKeyLaboratoryofUrbanWaterResourceandEnvironment(HarbinInstituteofTechnology),Harbin150090,China;2.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China)Abstract:TostudytheeffectsofwormpredationonmembranefoulinginanMBR+wormreactor,anMBR+wormreactorwithworm(R1)andanMBR+wormreactorwithoutWOrlTI(R2)wereoperatedinparalle1.Variationoftransmembranepressure(PTM)andmicrobialmetaboliteswerestudiedintheMBR(S—MBR)oftheR1andtheMBR(C—MBR)oftheR2.Denaturedgradientgelelectrophoresis(DGGE)wasusedforanalyzingthecompositionanddistributionofmicrobialcommunityintheS—MBRandtheC—MBR.TheresultsshowedthatthemembranefoulingcyclesoftheS—MBRandtheC—MBRwere90dand28-37d.respectively.Wormpredationdecreasedthepolysaccharideandproteinsinthesolublemicrobialproducts(SMP)andextracellularpolymericsubstances(EPS)oftheS—MBR.haproteobacterium,Betaproteobacterium,Deltaproteobacterium,GeobacteronthemembranewiresurfaceoftheS—MBRandAzorhizobium,肋odobacter.Gammaproteobacterium,FlavobacteriaonthemembranewiresurfaceoftheC—MBRplayedanimportantroleinthemembranefouling.Caldilineawa
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