纳米银颗粒对膜生物反应器运行性能及硝化细菌丰度的影响

净水技术2020,39(1):109-113WaterPurificationTechnology扫我试试?李侃,赵群英,赵平歌.纳米银颗粒对膜生物反应器运行性能及硝化细菌丰度的影响[J].净水技术,2020,39(1):109-113.LIK,ZHAOQY,ZHAOPG.ImpactofsilvernanoparticlesonperformanceofMBRoperationandabundanceofnitrobacteria[J].WaterPurificationTechnology,2020,39(1):109-113.纳米银颗粒对膜生物反应器运行性能及硝化细菌丰度的影响李侃,赵群英,赵平歌(西安工业大学建工学院,陕西西安710021)摘要考察了0.10mg/L纳米银对膜生物反应器(MBR)运行、氨氧化菌(AOB)和亚硝酸盐氧化细菌(NOB)丰度的影响。运行试验结果表明,在投放纳米银前后,处理系统对化学需氧量(COD)平均去除率为95.4%和94.1%,处理水中的总氮(TN)以硝态氮为主,硝态氮平均质量浓度前后分别为12.5mg/L和13.2mg/L,因此,在纳米银颗粒影响下,MBR仍能达到普通MBR的处理效果。实时定量聚合酶链反应(QPCR)结果表明:在整个运行周期内,AOB与NOB菌群的基因数量都处于107数量级,且硝化螺旋菌Nitrospira为优势NOB;结合银离子的变化规律可知,纳米银大部分富集到污泥中,但硝化菌群的丰度没有受到长期投放纳米银颗粒的影响。关键词膜生物反应器(MBR)纳米银颗粒氨氧化细菌(AOB)亚硝酸盐氧化细菌(NOB)实时定量PCR(QPCR)中图分类号:TU992.3文献标识码:A文章编号:1009-0177(2020)01-0109-05DOI:10.15890/j.cnki.jsjs.2020.01.019ImpactofSilverNanoparticlesonPerformanceofMBROperationandAbundanceofNitro-bacteriaLIKan,ZHAOQunying,ZHAOPingge(InstituteofArchitectureandCivilEngineering,Xi′anTechnologyUniversity,Xi′an710021,China)AbstractTheinfluencesoflowconcentrationofsilvernanoparticles(0.10mg/L)ontheperformanceofmembranebioreactors(MBR),populationstructureanddynamicsofammonia-oxidizingbacteria(AOB)andnitrite-oxidizingbacteria(NOB)wasinvestiga-ted.Theresultsindicatedthatbeforeandaftertheadditionofsilvernanoparticles,removalefficienciesoftheprocessforchemicaloxy-gendemand(COD)hadbeenreached95.4%and94.1%,respectively.Totalnitrogen(TN)inthetreatedwaterweremainlyintheformofnitratenitrogen,whiletheconcentrationofNO-3-Nbeforeandafternano-sivlerloadingwere12.5mg/Land13.2mg/L.MBRcouldachievethesameeffectofcommonMBRontheinfluenceofsilvernanoparticles.Real-timeQPCRresultsshowedthattheabun-danceofAOBandNOBwereonthesameorderof107,andNitrospirawasthedominantspeciesofNOB.ResultsshowedthatcombinedwithAg+changerule,silvernanoparticleswereconcentratedinthesludge,buttheabundanceofnitrifyingbacterialwasnotimpactedbycontinuouslong-termsilvernanoparticlesloadingatthelowconcentrationofsilver.Keywordsmembranebioreactor(MBR)silvernanoparticlesa