在线化学清洗对厌氧MBR产甲烷性能影响研究

中国环境科学2017,37(9)：3339~3345ChinaEnvironmentalScience在线化学清洗对厌氧MBR产甲烷性能影响研究虞雪晴,王志伟*,梅晓洁,苗妍,吴志超(同济大学环境科学与工程学院,污染控制与资源化研究国家重点实验室,上海200092)摘要：研究了厌氧膜-生物反应器(AnMBR)污泥在不同浓度的NaOH(10~400mg/L)和NaClO(5~40mg/L)短期暴露下产甲烷活性(SMA)、产甲烷潜能(BMP)、脱氢酶活(DHA)和辅酶F420活性的变化情况.结果表明,采用NaOH作为清洗剂时,SMA随着NaOH浓度的增加而下降;当NaOH浓度在200mg/L内,厌氧微生物产甲烷活性受抑制但未致死,当NaOH浓度超过200mg/L时,产甲烷微生物活性降低甚至致死.从相关酶活性来看,DHA对NaOH具有耐受性,而辅酶F420在一定NaOH浓度范围内(10~100mg/L)受激发而活性增强,超过100mg/L其活性下降.当采用NaClO作清洗剂时,随着NaClO浓度的增加(0~40mg/L),SMA由50mLCH4/(gVSS·d)下降至15mLCH4/(gVSS·d);BMP在这一浓度范围内无明显变化;DHA和辅酶F420活性均随NaClO浓度的升高而降低,NaClO浓度为40mg/L时,DHA降低30%,辅酶F420活性降低70%.关键词：氢氧化钠；次氯酸钠；产甲烷活性；膜清洗；厌氧膜-生物反应器中图分类号：X703文献标识码：A文章编号：1000-6923(2017)09-3339-07Effectsofin-situchemicalcleaningonmethanogenicactivitiesinanaerobicmembranebioreactors.YUXue-qing,WANGZhi-wei*,MEIXiao-jie,MIAOYan,WUZhi-chao(StateKeyLaboratoryofPollutionControlandResourceReuse,CollegeofEnvironmentalScienceandEngineering,TongjiUniversity,Shanghai200092,China).ChinaEnvironmentalScience,2017,37(9)：3339~3345Abstract：NaOHandNaClOarethecommonchemicalsusedforin-situcleaninginanaerobicmembranebioreactors(AnMBRs);however,thecleaningreagentscanaffecttheviabilityofanaerobicmicrobesalongwiththeeliminationofmembranefoulants.Inthisstudy,themethanogenicactivitiesandrelatedenzymesofanaerobicsludgeweretestedundertheshort-termexposuretoNaOH(10~400mg/L)andNaClO(5~40mg/L),includingspecificmethanogenicactivity(SMA),biochemicalmethanepotential(BMP),dehydrogenaseactivity(DHA)andcoenzymeF420activity.TheresultsshowedthatSMAdecreasedwiththeincreaseofNaOHconcentration.TheNaOHconcentrationsof0~200mg/Lcausedinhibitioneffectswhiletheconcentrationsabove200mg/Lmightinducecelldeath.Intermsofkeyenzymes,dehydrogenasecouldtoleraterelativelyhighNaOHconcentrationswhilecoenzymeF420wasmoresensitivetoNaOH.TheactivitiesofcoenzymeF420wereslightlyimprovedattheNaOHconcentrationof10~100mg/LanddeclinedwhenNaOHconcentrationwasabove100mg/L.UndertheexposureofNaClO,SMAdecreasedfrom50mLCH4/(gVSS·d)to15mLCH4/(gVSS·d)whenNaOClconcentrationincre