厌氧反硝化产甲烷体系中喹啉与吲哚共基质的降解特性

中国环境科学2019,39(10)：4150~4156ChinaEnvironmentalScience厌氧反硝化产甲烷体系中喹啉与吲哚共基质的降解特性高艳娟,岳秀萍*,段燕青,张智春,张潇,罗艳红(太原理工大学环境科学与工程学院,山西太原030024)摘要：研究了厌氧反硝化产甲烷体系中,典型含氮杂环化合物喹啉、吲哚作为共基质碳源,厌氧生物对二者的降解特性,及群落分析.结果表明:在共基质条件下,喹啉的存在对吲哚的生物降解有抑制作用,且抑制随喹啉浓度的升高而升高;吲哚的存在对喹啉的生物降解有促进作用,但吲哚浓度过高(150mg/L)抑制了喹啉的降解;喹啉、吲哚共基质时,二者的降解都遵循零级反应动力学;通过GC-MS分析,喹啉的主要中间代谢产物分别为2(1H)喹诺酮与8-羟基-2(1H)喹诺酮;吲哚的主要代谢产物为2-吲哚酮与靛红;通过高通量测序对共基质体系的微生物群落进行分析,发现厌氧功能菌群得到富集,细菌菌门以变形菌门Proteobacteria为主,菌纲以Gammaproteobacteria和Betaproteobacteria为主,菌属以Acinetobacter,Candidimonas,Azospira,和Desulfomicrobium为主.关键词：厌氧；反硝化产甲烷；共基质；中间产物；微生物群落中图分类号：X703文献标识码：A文章编号：1000-6923(2019)10-4150-07Degradationofquinolineandindoleco-substrateunderanaerobicdenitrificationandmethanogenesisconditions.GAOYan-juan,YUEXiu-ping*,DUANYan-qing,ZHANGZhi-chun,ZHANGXiao,LUOYan-hong(CollegeofEnvironmentalScienceandEngineering,TaiyuanUniversityofTechnology,Taiyuan030024,China).ChinaEnvironmentalScience,2019,39(10)：4150~4156Abstract：Bathexperimrntswereconductedtostudythedegradationcharacteristicsandmicrobialcommunityofquinolineandindolesco-substrateunderanaerobicdenitrificationandmethanogenesisconditions,whichwereknownastypicalN-heterocycliccompounds.Theresultsshowedthatthepresenceofquinolonecouldinhibitthedegradationofindole,andtheinhibitioneffectwasenhancedwiththeincreaseofquinolineconcentration;thepresenceofindolecouldpromotethedegradationofquinoline,butthehighconcentrationofindole(150mg/L)inhibitedthedegradationofquinolone;thekineticsofquinolineandindolewasfollowedthezero-orderkineticsmodel;throughGC-MSanalyses,theintermediatemetabolitesofquinolinewere2-hydroxyquinolineand2,8-dihydroxyquinoline;andmetabolitesofindolewereoxindoleandisatin.Thehigh-throughputsequencingtechnologywasusedtoanalyzethemicrobialcommunitystructure,andresultsindicatedthatthefunctionalbacterialwereenrichedintheanaerobicdenitrificationandmethanogenesissystem.ThebacterialphylumwasProteobacteria,thedominantclasseswereGammaproteobacteriaandBetaproteobacteria,andthedominantgenerawereAcinetobacter,Candidimon