厌氧发酵液中微好氧功能菌的分离与鉴定

第4O卷第4期2018年8月三峡大学学报(自然科学版)JofChinaThreeGorgesUniv．(NaturalSciences)Vo1．40No．4Aug．2018DOI：10．13393／j．cnki．issn．1672—948X．2018．04．022厌氧发酵液中微好氧功能菌的分离与鉴定龚艳丽李珊珊。李宁吕育财郭金玲龚大春田毅红(1．三峡大学生物与制药学院，湖北宜昌443002；2．三峡大学水利与环境学院，湖北宜昌443002)摘要：在发酵前期，体系中微好氧微生物可以消耗掉氧气，为了给产甲烷菌提供一个厌氧环境，该研究的是甲烷发酵前期的微好氧微生物，通过实验，分离纯化得到3株纯功能菌1、3和4号菌；对其革兰氏染色发现均为革兰氏阳性菌；找到其最适生长温度为40~C及pH为7；对其生长曲线进行测定得出，1、3和4号菌的生长周期分别为2d、2d和1d；测得不同碳源浓度，不同菌株的生长情况也有极大区别，三株菌的相似之处是对淀粉的分解利用率极高，对麦芽糖的分离利用率低．对于甲烷发酵这个复合体系，前两个阶段微好氧功能菌的耗氧作用对第三阶段产甲烷至关重要．因此，对微生物进行分离，获得厌氧消化体系内微好氧微生物的纯培养，深入研究功能菌株的相关性质，对进一步提高甲烷发酵效率具有重要意义．关键词：微好氧微生物；碳源；耐受度；分离中图分类号：TQ92：Q819文献标识码：A文章编号：1672—948X(2018)04—0103—05ResearchonMicro—aerobicFunctionalBacteriaSeparationandCharacteristicsofOrganicWasteMethaneFermentationGongYanliLiShanshanLiNingLvYucaiGuoJinlingGongDachunTianYihong(1．CollegeofBiological&PharmaceuticalScience，ChinaThreeGorgesUniv．，Yichang443002，China；2．CollegeofHydraulic&EnvironmentalEngineering，ChinaThreeGorgesUniv．，Yichang443002，China)AbstractIntheearlystageoffermentation，microaerobicmicroorganismsinthesystemcanconsumeoxy—gen．Inordertoprovideananaerobicenvironmentformethanogens，thisstudyis8micro—aerobicmicroorgan—ismintheearlystageofmethanefermentation．Byexperiment，threepurefunctionalbacteria1，3and4；Gram—positivebacteriawerefoundforGramstain；theoptimumgrowthtemperaturewasfoundtobe40℃andpH7；thegrowthcurvewasmeasured，4strainsofbacteriagrowthcyclewere2days，2daysand1day；measureddifferentcarbonsourceconcentrations，differentstrainsofthegrowthsituationisalsoverydiffer—ent，similartothethreestrainsofstarchdecompositionratefortheuseofHigh，formaltoseseparationandutilizationislow．Forthecomplexsystemofmethanefermentation，theoxygenconsumptionofmicroaerobicfunctionalbacteriainthefirsttwostagesisveryimportantforthethirdstageofmethaneproduction．There—fore，itisofgreatsignificancetofurtherimprovetheefficiencyofmethanefermentationbytheisolationofmicroorganismsandthepurecultureofmicroaerobicmicroorganismsintheanaerobicdigestionsystem．Keywordsmicroaerobicmicroorganism；carbonsou