一株好氧反硝化菌的同步脱氮降解苯胺特性

一株好氧反硝化菌的同步脱氮降解苯胺特性赵芝清1袁2袁黄乐3袁邱唯一1袁沈舒雯1袁熊萍1渊1.衢州学院化学与材料工程学院袁浙江衢州324000曰2.浙江大学环境与资源学院袁浙江杭州310018曰3.浙江工商大学环境科学与工程学院袁浙江杭州310018冤咱摘要暂采用批式实验探究了碳源尧抗生素尧重金属对好氧反硝化苯胺降解菌株同步脱氮降解苯胺特性的影响遥结果表明院经生理生化反应和16SrDNA测序袁鉴定为不动杆菌属渊命名为Acinetobactersp.H3冤遥在30益尧90r/min振荡培养条件下袁异养硝化率尧TN去除率尧苯胺降解率最大分别达57.98%尧54.24%尧100%遥在35滋g/L抗生素和80mg/L重金属胁迫下袁苯胺仍能完全转化降解袁好氧反硝化则受到了不同程度的抑制遥咱关键词暂Acinetobactersp.H3曰好氧反硝化曰苯胺降解咱中图分类号暂X172曰X703咱文献标识码暂A咱文章编号暂1005-829X渊2019冤07-0014-05SimultaneousanilinedegradationandaerobicdenitrificationcharacteristicsofanaerobicdenitrifierZhaoZhiqing1袁2袁HuangLe3袁QiuWeiyi1袁ShenShuwen1袁XiongPing1渊1.CollegeofChemical&MaterialEngineering袁QuzhouUniversity袁Quzhou324000袁China曰2.CollegeofEnvironment&ResourceSciences袁ZhejiangUniversity袁Hangzhou310018袁China曰3.SchoolofEnvironmentalScienceandEngineering袁ZhejiangGongshangUniversity袁Hangzhou310018袁China冤Abstract院Batchexperimentswereconductedtoinvestigatetheeffectsofcarbonsource袁antibioticsandheavymetalsonthecharacteristicsofthesimultaneousaerobicdenitrificationandanilinedegradationofanaerobicdenitrifier.TheresultsshowedthatitwasidentifiedasamemberofthegenusAcinetobacter渊namedAcinetobactersp.H3冤byphysio鄄logicalandbiochemicalreactionandits16SrDNAgenesequenceanalysis.Undertheshakingcultureconditionsof30毅Cand90r/min袁theheterotrophicnitrificationrate袁TNremovalrateandanilinedegradationratereached57.98%袁54.24%and100%袁respectively.Underthestressof35滋g/Lantibioticsand80mg/Lheavymetals袁anilinecouldstillbecompletelytransformedanddegraded袁andaerobicdenitrificationwasinhibitedtovaryingdegrees.Keywords院Acinetobactersp.H3曰aerobicdenitrification曰anilinedegradation近些年来袁好氧反硝化菌因其在脱氮过程中无需回流尧酸碱中和等诸多优点袁受到了广泛的关注遥1988年袁Robertson等首次报道了Thiosphaerapanto鄄tropha具有同时硝化反硝化功能袁并提出了异养硝化-好氧反硝化渊HN-AD冤菌的概念也1页遥至今袁国内外学者已筛选分离得到的好氧硝化菌主要有芽孢杆菌属渊Bacillus冤尧不动杆菌属渊Acinetobacter冤尧产碱杆菌属渊Alcaligenes冤尧微枝杆菌属渊Microvirgula冤尧假单胞菌属渊Pseudomonas冤等也2-7页遥为推进HN-AD技术在实验废水中的应用袁特殊环境下的研究成为了当前学者广泛关注的热点遥研究发现袁一些HN-AD菌株能够耐受贫营养渊碳质量浓度为1~15mg/L冤也8页尧低温和重金属等特殊环境也9-11页袁甚至有些HN-AD菌株还能够以苯酚作为唯一碳源也6袁12页遥苯胺是一种重要的化工原料袁被广泛应用于国防尧农药和医