一株好氧反硝化细菌的筛选及对土壤脱氮效果研究

中国麈擘c星提2018，34(20)：55—62ChineseAgriculturalScienceBulletin一株好氧反硝化细菌的筛选及对土壤脱氮效果研究陈寒玉。，靳慧征，靳慧霞(河南工业大学化学化工与环境学院，郑州450052；2郑州建筑职业技术学院工程管理系，郑州450000；浙江大学宁波理工学院，浙江宁波315100)摘要：为了探究反硝化生物茵剂于盐渍化土壤修复的应用机制和条件，以好氧反硝化能力为主要指标，以宁波慈溪地区的大棚种植土壤为样本，通过筛选比较进行菌株分离，得到一株高效好氧反硝化茵DPT001。通过形态、生理生化特征和16SDNA基因序列分析对菌种进行了茵属鉴定，并研究了该菌株适宜的生长条件。筛分出的好氧反硝化茵为革兰氏阳性短杆菌，属于克伯氏茵属的Castellaniella脱氮杆菌。在好氧环境下，52h时NO3降解率达到92％。菌株的适宜生长条件为中性或弱碱性土壤，盆栽试验表明，以此菌剂制备的生物茵肥(培养的菌种和腐熟花生壳混合)对土壤总盐度和硝酸盐浓度都有明显的降低作用。本研究从大棚土中筛选出的好氧反硝化茵，具有较高的反硝化特性，并优于市售的商品脱氮生物茵剂。关键词：土壤次生盐渍化；好氧反硝化；茵落特征；生物茵肥；土壤生物修复中图分类号：X172文献标志码：A论文编号：casbl7070006ScreeningofanAerobicDenitrificationStrainandItsNitrogenRemovalCharacteristicsforSoilChenHanyu，JinHuizheng2，JinHuixia(SchoolofChemistry,ChemicalandEnvironmentEngineering，HenanUniversityofTechnology，Zhengzhou450052；2HenanTechnicalCollegeofConstruction，Zhengzhou450000；NingboInstituteofTechnology,ZhejiangUniversity，Ningbo315100)Abstract：Toexploretheapplicationmechanismandconditionofdenitrifyingbiologicalagentinsalinesoilremediation，aerobicdenitrificationcapacitywasusedasthemainindex，theplantingsoilofgreenhouseinNingboCixiwasusedasthematerial，thebacterialstrainwasisolatedbyscreeningandcomparing．AnaerobicbacterialstrainwiththehighestactivitieswasobtainedandnamedDPT001．Thestrainwasidentifiedaccordingtoitsmorphological，physiologicalandbiochemicalpropertiesand16SDNAgenesequenceanalysis．Thesuitablecuhureconditionofthisstrainwasstudied．TheresultsshowedthatDPT001wasagram—positivebacillusandwasidentifiedasCastellaniellaginsengisoli．Intheanaerobicenvironment，NO3一degradationratewas92％in52h．Thesuitablegrowthconditionswereneutralorweakalkalinesoil．Thepotexperimentshowedthatthebiologicalbacterialfertilizer(mixedwiththestrainanddecomposedpeanutshel1)obviouslydecreasedthetotalsaltconcentrationandNO3一concentration．Anaerobicdenitrifyingstrainwasisolatedfromgreenhousesoilanditexhibitedhighdenitrificationcharacteristicsandwasbetterthanthecommercialdenitrificationbiologicalbacterialagent．Keywords：secondarysoilsalinizafion；aerobicdeni