木薯酒精废水脱色菌的筛选及脱色条件研究

安徽农业科学，JournalofAnhuiAgri．Sci．2010，38(33)：19060J19062责任编辑高晓余责任校对李岩木薯酒精废水脱色菌的筛选及脱色条件研究齐烁，浦跃武(华南理工大学生物科学与工程学院，广东广州51o0o6)摘要[目的]筛选出高效的木薯酒精废水脱色菌，并探索其最佳脱色条件。[方法]分别从华南理工大学环境学院、猎德污水处理厂取得污泥，经过分离纯化和初步筛选，得到对木薯酒精废水具有脱色作用、且脱色效果稳定的1株菌株，并通过单因素试验研究以其作为出发菌的最佳脱色条件。[结果]分离纯化和初步筛选结果表明，猎德污水处理厂的9号菌脱色效率最高，为20．3％；以9号菌作为出发菌的脱色单因素试验结果表明，9号菌的最佳接种和脱色时间分别为16h和24h；9号菌最佳脱色条件为：以葡萄糖为外加碳源，以NaNO，为外加氮源，好氧条件，温度30℃；在该最佳条件下，其脱色能力达29．6％。[结论]单一菌种的脱色能力有限，该研究为获得脱色能力较好的复合菌群奠定了基础。关键词木薯酒精废水；脱色茵；条件；优化中图分类号$533文献标识码A文章编号0517—6611(2010)33—19060—03ScreeningofCassavaAlcoholWastewater．decolorizingMicrobeandConditionsofDecolorizationQIShuoetal(SchoolofBioscienceandBioengineering，SouthChinaUniversityofTechnology，Guangdong，Guangdong510006)Abstract10biectiveIThepurposeofthisstudywastoobtainanefficientcassavaalcoholwastewater．deeolorizingmicrobe，thentofindoutthebestdecolorizationconditions．IMethodlSludgewasacquiredfrolnSchoolofEnvironment，SouthChinaUniversityofTechnologyandLiedesewagetreatmentplant．Througheffectivescreening，amicro—organismwithstrongandstabilizedecolorizationabilityforwastewatertreatmentwasacquired．Thenthebestdecolorizationconditionsofthiswastewater—decolorizingnficrobewerestudiedthroughthesinglefactorexperiments．『Resuh]rheisolatingandpurifyingexperimentresultsshowedthatthedecolorationeficiencyoftheninthmicrobefromLiedesewagetreatmentplantwasthehighestinal】themicrobes．reached2O．3％．Theoptimalvaccinationanddecolorizationtimeoftheninthmi—erobewas16hand24hrespectively．ThesinglefactorexperimentsresultsshowedthatGlucoseandNaNO。werethebestcabonandnitrogensourcesrespectively：themicroorganismwouldbefedwithaerobictreatmentin30oC，thedecolorizationeficiencywasupto29．6％．1Con—clusion1Wecanconcludethatthedecolorizationabilityofasinglespecieswaslimited：theseprimaryresearchresultsmightprovidethefoun—dati0nfortheacquirementofthemixturestrainswithbetterdecolorizationability，KeywordsCassavawastewater：Decolorizationbacteria：Condition：Optimization木薯生物学产量大、淀粉含量高。木薯的块根含30％的淀粉，木薯干则含有70％的淀粉，被誉为“淀粉之王”，是很有前途的酒精生产原料⋯。目前，我国酒精产量的1／3以木薯为