垃圾渗滤液处理菌株的筛选及处理工艺条件的优化

垃圾渗滤液处理菌株的筛选及处理工艺条件的优化贺晓凌1袁赵丹瑜2袁张佩莲2袁向雪艳2袁李卓轩2袁魏东盛3渊1.天津工业大学化学与化工学院袁天津300387曰2.天津工业大学环境科学与工程学院袁天津300387曰3.南开大学生命科学学院袁天津300071冤ScreeningofstrainsforlandfillleachatetreatmentandoptimizationoftreatmentconditionsHEXiao-ling1袁ZHAODan-yu2袁ZHANGPei-lian2袁XIANGXue-yan2袁LIZhuo-xuan2袁WEIDong-sheng3渊1.SchoolofChemistryandChemicalEngineering袁TiangongUniversity袁Tianjin300387袁China曰2.SchoolofEnvironmen原talScienceandEngineering袁TiangongUniversity袁Tianjin300387袁China曰3.SchoolofLifeSciences袁NankaiUniversi原ty袁Tianjin300071袁China冤Abstract：Inordertoimprovetheleachatepollutioncausedbylandfilldisposalofmunicipalsolidwaste袁thesoilsamplescollectedfromsomewastesanitarylandfillwereusedasthesourceofbacteriatoscreenthedominantstrainsuit鄄ablefordegradinglandfillleachate.Plackett-Burman渊PB冤experimentwasusedtoscreenthemainfactorsaf鄄fectingthedegradationoflandfillleachatebystrains.ThenthepathofsteepestascentwastakentoapproachtheoptimalresponsesurfaceareaofdegradationconditionsbasedontheresultsofPBexperiment.Finally袁theopti鄄maldegradationconditionswereobtainedbytheresponsesurfacemethod渊RSM冤.Theresultsshowthatthedom鄄inantstrainisidentifiedasFusariumchlamydosporumbymolecularbiologyandnamedasFusariumchlamy鄄dosporumTJPU02.ThreemainfactorsaffectingthedegradationoflandfillleachatebyFusariumchlamydosporumTJPU02arepeptoneconcentration袁inoculumsizeandglucoseconcentration.Theoptimaldegradationconditionsarepeptone4.3mg/mL袁inoculumsize6.44%袁glucose9.2mg/mL.ThemeanvalueofCODdegradationrateob鄄tainedfromtheverificationexperimentsis85.76%undertheseoptimalconditions袁whichisingoodagreementwiththetheoreticalpredictedvalueof85.95%.ItillustratesthatthedominantstrainFusariumchlamydosporumTJPU02exhibitessignificantdegradationeffectonlandfillleachateandhasbroadapplicationprospects.Keywords：landfillleachate曰Fusariumchlamydosporum曰responsesurfacemethod曰CODdegradationrate曰processop原timization摘要：为了改善城市生活垃圾经填埋处理产生的渗滤液污染问题袁以某生活垃圾卫生填埋场采集的土样作为菌源袁筛选出适于降解垃圾渗滤液的优势菌株袁采用Plackett-Burman实验设计法筛选出影响菌株降解垃圾渗滤液的主要因素袁在此基础上运用最陡坡实验法接近最大响应值区域袁通过