垃圾渗滤液浸种对玉米代际遗传效应的研究
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2020-04-09 14:20:07
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2016年第11卷第4期。108—113生态毒理学报AsianJournalofEcotoxicologyVo1.11,2016No_4.108.113DOI:10.7524/AJE.1673—5897.2016033l009李广科,陈俊艳.垃圾渗滤液浸种对玉米代际遗传效应的研究⋯.生态毒理学报,2016,1l(4):108—1l3LiGK,ChenJTheinter—generationalgeneticeffectofZeamay$L.(maize)inducedbysoakingwithlandfillleachate[J].AsianJournalofEcotoxicol-ogy,2016,l1(4):108一l13(inChinese)垃圾渗滤液浸种对玉米代际遗传效应的研究李广科,陈俊艳山西大学环境与资源学院.太原030006收稿日期:2016.03—31录用日期:2016—04.11摘要:采用实验室早期栽培实验,主要探讨了垃圾渗滤液浸种对玉米代际的遗传效应结果表明:垃圾渗滤液会影响玉米代际早期的生长发育,低浓度垃圾渗滤液(1O%)促进代际玉米种子的萌发及幼苗的生长;而高浓度渗滤液(50%)则对其产生抑制效应。再者,相比于经垃圾渗滤液浸种处理过的F代玉米种子而言,对F,代产生的毒性效应相对减弱.仅高浓度组与对照组相比出现差异,50%处理组的萌发率、根长、芽长及叶绿素含量分别为对照组的81%,83%,79%和89%。此外,渗滤液浸种对代际玉米根尖细胞有丝分裂均表现出“低促高抑”的作用,对根尖微核损伤也均呈现剂量.效应关系。这说明低浓度垃圾渗滤液对玉米F,代及F代的生态毒性风险依然存在,不容忽视。研究选用玉米为模式植物具有一定的实际意义,其结果可为垃圾渗滤液的生态风险评价提供理论依据。关键词:垃圾渗滤液;玉米;遗传效应;浸种文章编号:1673—5897(2016)4—108—06中图分类号:X171.5文献标识码:ATheInter—generationalGeneticEfectofZeamaysL.(Maize)InducedbySoakingwithLandfillLeachateLiGuangke,ChenJunyanCollegeofEnvironmentalScienceandResources,ShanxiUniversity,Taiyuan030006,ChinaReceived31March2016accepted11April2016Abstract:Thepresentresearchmainlyinvestigatedthegenerationalgeneticefectofthemaizeseedinducedbysoakingwithlandfillleachatebythemethodoftheearlycultivationexperiment.Theresultsshowedthatleachatewithdiferentlevels(0%,2%,10%,20%,30%,50%)afecttheearlygrowthanddevelopmentofgenerationalmaize.Lowconcentrations(10%)ofleachatepromotedseedgerminationandseedlinggrowth,whilehighconcen-trations(50%)showedrestrainedefects.Moreover,thetoxicityefectonF2generationofmaizewasrelativelyweakenedcomparedwiththeFlafterseedsoakingwithleachate,andthesignificantdiferencesofF2wereonlyobservedbetweenhighconcentrationgroupandcontrolgroup.Thegerminationrate,rootlength,shootlengthandchlorophyllcontentof50%treatmentgroupwere81%,83%,79%and89%ofthecontrolgroup,respectively.Furthermore,theinfluenceofleachateonroottipmitoticindexshowedthatlOWconcentrationpromotedmitosisandhighconcentrationsuppressedit,whilethemicronucleusdamagepresentedadose.efectrelationship.There-基金项目:国家自
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