4种兼性厌氧微生物农药的芽孢含量检测方法研究

PesticideScienceandAdministration2019,40(1)4种兼性厌氧微生物农药的算抱食量检测方法研究王双，师丽红，袁善奎*,张燕(农业农村部农药检定所，北京100125)StudyontheActiveIngredientDeterminationMethodsfor4FacultativeAnaerobicMicrobialPesticidesWangShuangShiLihong,YuanShankui,ZhanYan(InstitutefortheControlofAgrochemi­cals,MinistiyofAgriculture&RuralAffairs,Beijing100125,China)Abstract:Thisstudyfocusedonfourfacultativeanaerobicmicrobialpesticides,includingBacillusmethylotrophicus,Paenibacilhispolymyxa,BrevibacilhislaterosponisandBacillusamyloliquefaciens.Todevelopthemostappropriatemethodsfordeterminingthesporecontent,threeculturalmethodswithdif­ferentculturalmediawereselected,whichwereaimedtoprovidereferenceforqualitytestofmicrobialpesticides.Theresultsshowedthatnutrientagar(NA)wasthebestmediumforthedeterminationoftheother3microbialsthanBacillusamyloliquffaciens,forwhichNo.4mediumwasrecommendedtobeused.Undertheoptimumculturemedium,thethreeculturemethodswerecomparedandanalyzed.TheresultsdisplayedthatahigheractiveingredientcontentofBacillusinethylotrophicusandBrevibacilluslal-erosporuswasobtainedwhensandwichplatemethodandpourplatemethodwereused,andthatsignifi­cantlyhigherresultswereobtainedforPaenibacilluspolymyxaandBacillusamyloliquefacienswhenpourplatemethodwasusedcomparedtoplatemethodandsmearplatemethod.Keywords:facultativeanaerobic;microbialpesticides；activeingredientdetermination；culturalmedia；culturalmethods摘要：本文以甲基营养型芽葩杆菌、多粘类芽胞杆菌、侧砲短芽胞杆菌、解淀粉芽胞杆菌等4种兼性厌氧微生物农药为材料，在不同培养基上用3种不同的培养方式进行芽胞含量检测收稿日期：2018-11-20作者简介：王双，女，硕士，主要从审生物农药产品质量检测工作。联系电话：18633626170；E-mail：wangshuang-jol)s@126.coni通讯作者：袁善奎，男，博士，研究员，主要从事农药登记管理工作。E-mail：skvuan76@sina.coino—29—PesticideScienceandAdministration2019,40(1)以探究最佳的检测方法，为微生物农药质量检测提供参考。结果表明，除解淀粉芽砲杆菌外,营养琼脂（Nutrientagar,NA）是其他3种兼性厌氧微生物农药芽胞含量检测的最佳培养基,而解淀粉芽砲杆菌进行芽胞含量检测时推荐使用四号培养基。在最佳培养基培养条件下，对3种培养方式进行比较分析，结果表明对于甲基营养型芽胞杆菌和侧砲短芽胞杆菌，平皿夹层法和倒平皿法有效成分含量检测结果较高，而多粘类芽胞杆菌和解淀粉芽胞杆菌倒平皿法检测结果极显著高于平皿夹层法和涂平皿法（Duncan检验，PwO.01）。关键词：兼性厌氧；微生物农药；芽胞含量检测；培养基；培养方法中图分类号：S482.2文献标志码：A文章编号：1002-5480（2019）1-29-61引言有效成分含量是决定微生物农药药效的重要因