养殖废水中异养硝化细菌的分离筛选和鉴定

农业环境科学学报2010，29(11)：2232—2237JournalofAgro—EnvironmentScience养殖废水中异养硝化细菌的分离筛选和鉴定刘芳芳，一，周德平，吴淑杭，一，张明，褚长彬，范洁群，姜震方(1．华东师范大学环境科学系，上海200063；2．上海市农业科学院生态环境保护研究所，上海201106)摘要：为了获得脱氮功能强的异养硝化菌株用于养殖废水的脱氮处理，通过富集、分离和纯化等步骤，并结合格利斯试剂检验菌株硝化能力的方法，从某养猪场污水处理池污泥中分离筛选了4株具异养硝化功能的菌株，分别标号为79、84、L1l6、L117，通过16SrDNA序列分析和美国全自动微生物分析仪Biolog鉴定，4株菌均为粪产碱杆菌(A妇sfaecalis)，并验证了这4株菌的硝化能力。结果表明，当液体培养基初始氨氮浓度为90mg·L。左右时，在振荡培养48h内，菌株79、84、L116、L117培养基中氨氮和总氮均快速下降，氨氮去除率分别达到44．4％、47．9％、61．3％和56．4％，总氮(除菌)去除率达到39．9％、38．5％、43．4％和40．7％。关键词：养猪场废水；异养硝化细菌；分离；脱氮能力；粪产碱杆菌中图分类号：X172文献标志码：A文章编号：1672—2043(2010)11-2232—06IsolafioilandIdentificationofHeterotrophicNitrifiersfromCultivationWastewaterLIUFang-fangZHOUDe—ping~，WUShu-hang。ZHANGMing，CHUChang-bin。，FANJie-qun，JIANGZhen-fangz(1．DepartmentofEnvironmentScience，EastChinaNormalUniversity，Shanghai200063，China；2．InstituteofEco-EnvironmentalProtec-tion，ShanghaiAcademyofAgriculturalSciences，Shanghai201106，China)Abstract：Thebiologicalni~ogenremovaliswidelyappliedinthewastewatertreatment，anditismoreeconomicinallnitrogenremovalmethods．ThetraditionalbiologicalnitrogenremovalmetllodiSmainlydedicatedtotheresearchofautotrophicnitrobacteria．Comparedtotheautotrophicnitrobacteria，heterotrophicnitrificationbacteriaareabletoutilizeorganiccarbonsourcesandinorganiccarbonsources．WhatSmore，theyhaveabsolutesuperiorityinadaptivecapacityandcanwidelyexistintheenvironment．Inordertogettheheterotrophienitrifica—tionbacteriawhichcanremovenitrogenincultivationwastewater，fourstrainsofheterotrophicnitrifiersfromthesewageofanhoggeryinCongming(Shanghai)throughtheprocedureofenriching，separating，purifyingandidentificatingtheirnitrificationcapacitybytheprincipleofwhennitritemeetsEglisereagent，itwillbecomered．Thefourstrainsweregiventhelabelsof79，84，L116andL117．11}le16SrDNAse—quenceanalysisandtheUnitedStatesautomaticmicrobialanalyzerBiologwereusedtoidentifythestrains．TheresultshowedthatthefourstrainswereAIcdsfaecalis．Theresultofexperimentshowedthatwhentheinitialammonia-nitrigonconcentrationreached90mg。L一，thenit