_泥种对高盐同步脱氮除磷系统快速构建的影响
- 张立中
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2020-04-22 18:19:15
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泥种对高盐同步脱氮除磷系统快速构建的影响李晓品,周健,韩懿,陈浬,曾国明(重庆大学三峡库区生态环境教育部重点实验室,重庆400045)摘要:针对高盐条件下生物脱氮除磷系统构建周期长、构建困难的问题,在前期对高盐榨菜废水磷酸盐生物还原除磷及同步硝化反硝化脱氮技术研究的基础上,探讨高盐榨菜废水基于磷酸盐还原除磷的同步脱氮除磷系统的快速构建方法,重点考察不同城市污水厂的接种污泥对系统构建的影响,并采用PCR-DGGE技术探讨了系统微生物种群结构的变化。在盐度为3%(以NaCl计)、水温为(30±2)℃、DO为3~4mg/L、负荷为0.5kgCOD/(m3·d)的条件下,接种不同城市污水厂污泥的反应器在18~30d内均能够成功构建出同步生物脱氮除磷系统,对COD、PO3-4-P、NH+4-N、TN的去除率分别达到95%、60%、97%和95%以上,泥种对系统构建的影响不显著。PCR-DGGE研究表明:在系统构建过程中微生物群落结构发生了变化,但与接种污泥微生物种群仍存在56.4%的相似度,表明接种污泥中有部分微生物可以适应高盐环境。关键词:高盐废水;同步脱氮除磷;PCR-DGGE;生物系统构建中图分类号:X703文献标识码:A文章编号:1000-4602(2013)01-0014-05基金项目:国家水体污染控制与治理科技重大专项(2008ZX07315-004)EffectofInoculatedSludgeonEstablishmentofSimultaneousNitrogenandPhosphorusRemovalSystemLIXiao-pin,ZHOUJian,HANYi,CHENLi,ZENGGuo-ming(KeyLaboratoryofThreeGorgesReservoirRegion’sEco-Environment<MinistryofEducation>,ChongqingUniversity,Chongqing400045,China)Abstract:Toaddressthelongperiodanddifficultyinestablishingabiologicalnitrogenandphos-phorusremovalsystemunderhigh-salinitycondition,thequickestablishmentmethodofsimultaneousni-trogenandphosphorusremovalsystemforhigh-salinitymustardtuberwastewatertreatmentwasproposed.Theresearchwasbasedontechnologiesofphosphatebio-reductionandsimultaneousnitrificationanddenitrification.Theeffectofinoculatedsludgefromdifferentmunicipalwastewatertreatmentplantsontheestablishmentofthesystemwasinvestigated,andPCR-DGGEwasusedtostudythemicrobialcommunitystructure.Undertheconditionsof3%salinity(calculatedasNaCl),(30±2)℃temperature,3to4mg/LDOand0.5kgCOD/(m3·d)organicloading,thequickestablishmentofsimultaneousbiologicalnitrogenandphosphorusremovalsystemwasachievedwithin18to30dinthereactorsinoculatedwithsludgefromdifferentmunicipalwastewatertreatmentplants.TheremovalratesofCOD,PO3-4-P,am-monianitrogenandTNweremorethan95%,60%,97%and95%respectively.Theeffectofinoculatedsludgeontheestablishmentofthesystemwasnotremarkable.ThePCR-DGGEresultsshowedthatthemicrobialcommunitystructurewaschangedduringtheestablishmentofthesystem,buthadasimilarityof·41·第29卷第1期2013年1月中国给水排水CHINAWAT
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