污水 稳定塘菌-藻生态系统去除与灭活植物病毒TMV 研究

文章编号:025322468(2004)0621128207中图分类号:Q939,X71文献标识码:A污水稳定塘菌2藻生态系统去除与灭活植物病毒TMV研究郑耀通1,2,林奇英2,谢联辉2(1.福建农林大学资源与环境学院,福州350002;2.福建农林大学植物病毒研究所,福州350002)摘要:了解污水稳定塘生态系统去除与灭活植物病毒的效率与机理具有重要的理论和实际意义.对模型植物病毒———烟草花叶病毒(tobaccomosaicvirus,TMV)的实验结果显示:稳定塘系统中的悬浮固体(SS)可在短时间内吸附TMV且达到一个饱和值.纯培养的枯草芽孢杆菌及小球藻对TMV的存活无明显不良影响,而光合细菌和稳定塘中的混合细菌群却对TMV具有灭活作用.当它们同藻类形成菌藻共生体系时,对TMV的灭活速率分别从对照的每天010404log10(枯斑数·mL-1)升高到010783log10(枯斑数·mL-1)和01075log10(枯斑数·mL-1)1因此,在污水稳定塘生态系统中,由于病毒对悬浮固体的吸附以及菌藻共生体系中微生物的作用可有效地去除与灭活植物病毒,其中悬浮固体吸附病毒并非真正意义上的灭活,稳定塘中存在的细菌特别是光合细菌对植物病毒的灭活才是最主要的因素.关键词:烟草花叶病毒;稳定塘;病毒灭活;菌藻系统RemovalandinactivationofTMVbybacteria2algalecosysteminawastewaterstabilizationpondZHENGYaotong1,2,LINGQiying2,XIELianhui2(1.CollegeofResourcesandEnvironment,FujianAgricultureandForestUniversity,Fuzhou350002;2.InstituteofPlantVirus,FujianAgricultureandForestUniversity,Fuzhou)Abstract:Theresultsofremovalandinactivationoftobaccomosaicvirus(TMV),arepresentativevegetalvirus,inlaboratorialmodelsbyal2gal2bacteriaecosysteminasewagestabilizationpondwerestudied.Theresultsshowedthat:TMVcanberapidlyadsorbedtothesuspendsolidinstabilizationpond(SS)byareversibleprocess.TheproportionofadsorptionTMVwasdeterminedbythecharacteristicsoftheSS,andtherewasasaturationvalueoftheadsorptionTMVwitheverySSunit.Theresultsofthemicroorganisms,whichwerefoundmostlyinthebacteria2algalsystem,showed:ThepureculturesofBacillussubtitlesandChlorellavulgarisminsterilesewagehadnoeffectonTMVsurvival,where2as,thegrowthofphotosyntheticbacteria(PSB)andheterogeneouspopulationsofstabilizationpondbacteria(HSPB)inthesamemediumre2sultedinsubstantialvirusinactivation.TheabilityofPSBandHSPBfortheinactivationofTMVwouldbepromotedafterwhichtheywerecul2turedwithalgalandformedthebacteria2algalsymbiosissystem.TheactivityofPSBandHSPBwasthemostimportantfactorfortheinactivationofTMVinthesewagestabilizationpondsystems.Keywords:tobaccomosaicvirus;stabilizationpond;virusesinactivation;algal2bacteriasystem收稿日期:2003212206;修订日期:2004206224基金项目:福建省科技计划项目资助(982R217)作者简介:郑耀通(1965—),男,副教授(