_橡胶工业有机废水降解微生物的驯化筛选与降解条件研究

工业用水与废水INDUSTRIALWATER&WASTEWATERVol.37No.5Oct.,2006橡胶工业有机废水降解微生物的驯化筛选与降解条件研究摘要:用含M盐(2-硫醇基苯并噻唑)的橡胶工业有机废水驯化活性污泥,筛选出8株菌。纯化后进行混合培养,对其生长条件进行分析后发现:复合菌适合在30～35℃的偏碱性条件下生长,能耐受50g/L盐度的影响。当添加葡萄糖和尿素后对复合菌生长影响较大,同时可使橡胶工业有机废水降解率分别提高18.6%和7.56%;无机离子也对复合菌的降解能力有一定的促进作用。采用综合正交试验得出:葡萄糖、尿素、菌量、转速分别为2g/L、1g/L、20%、80r/min为复合菌的最佳降解条件,最高降解率为68.24%,从而提高了投菌法的降解效率。关键词:橡胶工业有机废水;驯化筛选;生物降解中图分类号:X703.1文献标识码:A文章编号:1009-2455(2006)05-0054-05ResearchonacclimationandscreeningofmicroorganismfordegradationoforganicwastewaterfromrubberindustryanddegradationconditionsthereofGuTao1,LIHan-dong2,WANGPing1(1.ChineseResearchAcademyofEnvironmentalScience,Beijing100012,China;2.SchoolofLifeScienceandTechnology,CentralSouthUniversityofForestandTechnology,Changsha412004,China)Abstract:8strainsofbacteriawereisolatedfromtheactivatedsludgeacclimatedbyMsalt(2-mercaptobenzo-thiazole)-containingorganicwastewaterfromrubberindustry.Thebacteriaweremixedcultivatedafterthepurifica-tion,throughanalyzingtheirlivingconditions,itwasfoundthat:compoundbacteriaadapttoalkalescentconditonat30-35℃,andtolerate50g/LNaCl.Addingglucoseandcarbamidegreatlyinfluencedthegrowthofthecompoundbacteria,andincreasedthedegradationrateofthewastewaterfromrubberindustryby18.6%and7.56%respec-tively;inorganicionscaneffectivelyimprovetheperformanceofthecompoundbacteriaonwastewaterdegradation,theresultsofthecomprehensiveorthogonalexperimentshowedthat,thehighestdegradationrateofwastewaterbythecompoundbacteriawas68.24%whichcouldbeobtainedwhenthemassconcentrationofglucose,carbamide,thebacteriacontent,were2g/L,1g/L,20%respectively,andtherevolutionspeedwas80r/min;theoptimalconditionindicatedabovewouldraisethebiodegradationvalueofthewastewaterbybacteria-throwingmethod.Keywords:organicwastewaterfromrubberindustry;acclimationandscreening;biodegradation顾韬1,2,李捍东1,王平2(1.中国环境科学研究院,北京100012;2.中南林业科技大学生命科学与技术学院,长沙412004)工业用水与废水INDUSTRIALWATER&WASTEWATERVol.37No.5Oct.,2006随着现代合成技术的发展,大量的异生化合物进入水体,其所具有的生物陌生性、毒性及化合物结构的复杂性,很难在短时间内被常规微生物利用进入物质循环,致使处理系统的水质恶化。橡胶工业生产中的促进剂M盐(2-硫醇